-transparent.png){kind=logo}
Microtiter solid-phase radioimmunoassay (micro-SPRIA) was applied to the detection of hepatitis B e antigen (HBeAg) in the serum. Antibody against HBeAg to coat the microtiter plate and for radiolabeling was obtained from the serum of asymptomatic carriers of hepatitis B surface antigen by an affinity column of partially purified HBeAg. The micro-SPRIA was sensitive and could be performed without prior concentration of the test serum. HBeAg was detected in 21 (35%) out of 60 serum samples positive for hepatitis B surface antigen, all of which were negative by the conventional immunodiffusion method even after they had been concentrated threefold.
Related Publications
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
October 1973,
Applied microbiology,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
February 1976,
Journal of immunology (Baltimore, Md. : 1950),
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
June 1973,
JAMA,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
April 1981,
The Journal of allergy and clinical immunology,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
December 1984,
Radioisotopes,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
March 1974,
Vox sanguinis,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
June 1973,
Lancet (London, England),
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
March 1982,
JAMA,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
October 1979,
Klinische Wochenschrift,
Y Miyakawa, and
F Tsuda, and
Y Akahane, and
M Mayumi
January 1975,
Transfusion,
