BIOMAT Database Logo BIOMAT Database Logo

A novel method for correlating internal and external structure of individual myelinated nerve fibres. 2001

Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Department of Neurology and University Department of Neurology, Royal Adelaide Hospital, Adelaide, Australia.

A new method is described that enables longitudinal and cross sections of an individual nerve fibre to be cut at multiple specified sites along the fibre by the use of a unique marker system. In this way the internal structure of the fibre can be correlated with the external appearance. Individual myelinated nerve fibres are teased apart in epoxy resin and mounted onto a carbon-coated slide, and after orientation and marking of specific sites of interest are snap frozen to liberate the orientated and marked fibres for embedding on the surface of an epoxy resin block for subsequent longitudinal or transverse sectioning. This method is particularly useful for the correlative study of the myelin-axon relationships.

UI MeSH Term Description Entries
D008867 Microtomy The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness. Thin Sectioning,Ultramicrotomy,Sectioning, Thin,Sectionings, Thin,Thin Sectionings
D009413 Nerve Fibers, Myelinated A class of nerve fibers as defined by their structure, specifically the nerve sheath arrangement. The AXONS of the myelinated nerve fibers are completely encased in a MYELIN SHEATH. They are fibers of relatively large and varied diameters. Their NEURAL CONDUCTION rates are faster than those of the unmyelinated nerve fibers (NERVE FIBERS, UNMYELINATED). Myelinated nerve fibers are present in somatic and autonomic nerves. A Fibers,B Fibers,Fiber, Myelinated Nerve,Fibers, Myelinated Nerve,Myelinated Nerve Fiber,Myelinated Nerve Fibers,Nerve Fiber, Myelinated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001369 Axons Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body. Axon
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D013497 Sural Nerve A branch of the tibial nerve which supplies sensory innervation to parts of the lower leg and foot. Nerve, Sural,Nerves, Sural,Sural Nerves
D016613 Plastic Embedding The infiltrating of histological specimens with plastics, including acrylic resins, epoxy resins and polyethylene glycol, for support of the tissues in preparation for sectioning with a microtome. Embedding, Plastic
D016616 Cryoultramicrotomy The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination. Cryosectioning,Freeze Sectioning,Sectioning, Freeze
D048429 Cell Size The quantity of volume or surface area of CELLS. Cell Volume,Cell Sizes,Cell Volumes,Size, Cell,Sizes, Cell,Volume, Cell,Volumes, Cell

Related Publications

Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Structure of normal peripheral myelinated nerve fibres.
January 1981, Advances in neurology,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Form of myelinated nerve fibres.
May 1954, Nature,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
A method for increasing the frequency response of voltage clamped myelinated nerve fibres.
June 1981, Pflugers Archiv : European journal of physiology,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
The fine structure and morphological organization of non-myelinated nerve fibres.
March 1956, Proceedings of the Royal Society of London. Series B, Biological sciences,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Familial myelinated retinal nerve fibres.
January 2003, Eye (London, England),
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Apparatus for measuring and counting myelinated nerve fibres.
August 1961, Journal of neurology, neurosurgery, and psychiatry,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
A model of myelinated nerve fibres for electrical prosthesis design.
July 1994, Medical & biological engineering & computing,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Imaging myelinated nerve fibres by confocal fluorescence microscopy: individual fibres in whole nerve trunks traced through multiple consecutive internodes.
September 1994, Journal of neurocytology,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Saltatory conduction in myelinated nerve fibres.
September 1952, The Journal of physiology,
Z Cai, and K Cash, and J Swift, and P Sutton-Smith, and P D Thompson, and P C Blumbergs
Potassium inactivation in myelinated nerve fibres.
April 1971, Experientia,
Copied contents to your clipboard!