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The stroma and epithelium of human corneas that had been stored in organ culture medium for 10 to 22 days at 37 C were examined by light and electron microscopy. Total corneal thickness was found to be doubled at ten days and there was no further increase even at 22 days. The posterior portion of the stroma was more hydrated than the anterior region. Stromal cells were reduced in number and normal-appearing cells were present only in superficial stroma. The epithelial basement membrane was irregular and thickened. Although the epithelium was reduced to three or four cells in thickness and the intercellular spaces were dilated, the epithelial cells contained normal subcellular organelles and appeared to be viable.
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
December 1962,
American journal of ophthalmology,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
May 1980,
Experimental eye research,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
May 2012,
Tissue engineering. Part C, Methods,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
January 1977,
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D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
January 1980,
Cell and tissue research,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
January 2008,
Scandinavian journal of gastroenterology,
D L Van Horn, and
D J Doughman, and
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Okajimas folia anatomica Japonica,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
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R A Good
January 1985,
Revista de chirurgie, oncologie, radiologie, o. r. l., oftalmologie, stomatologie. Seria: Oftalmologie,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
October 1974,
Journal of molecular and cellular cardiology,
D L Van Horn, and
D J Doughman, and
J E Harris, and
G E Miller, and
R Lindstrom, and
R A Good
February 1948,
Bulletin of the Johns Hopkins Hospital,
