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To achieve chondrocyte-specific deletion of floxed genes we generated a transgenic mouse line expressing the Cre recombinase under the control of the mouse type II collagen gene (Col2a1) regulatory regions. Northern and in situ hybridization analyses demonstrated the expression of the transgene (Col2a1-Cre) in cartilaginous tissues. To test the excision efficiency of Cre, the Col2a1-Cre strain was crossed with the ROSA26 reporter strain. LacZ staining of double transgenic mice revealed Cre activity in both chondrogenic and non-chondrogenic tissues. During early embryonic development (E9.5-11.5), LacZ expression was detected in tissues where the endogenous Col2a1 transcript is expressed such as the otic capsule, notochord, developing brain, sclerotome and mesenchymal condensations of future cartilage. At later stages, Cre activity was observed in all cartilaginous tissues with virtually 100% of chondrocytes being LacZ positive. These data suggest that the Col2a1-Cre mouse strain described here can be useful to achieve Cre-mediated recombination in Col2a1 expressing cells, especially in chondrocytes.
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
June 1998,
The Journal of cell biology,
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
February 2000,
Genesis (New York, N.Y. : 2000),
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
March 1995,
Human molecular genetics,
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
August 1990,
Proceedings of the National Academy of Sciences of the United States of America,
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
May 2004,
Genesis (New York, N.Y. : 2000),
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
January 1985,
Progress in clinical and biological research,
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
February 2000,
Genesis (New York, N.Y. : 2000),
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
January 2003,
Genesis (New York, N.Y. : 2000),
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
July 2004,
Genesis (New York, N.Y. : 2000),
K Sakai, and
L Hiripi, and
V Glumoff, and
O Brandau, and
R Eerola, and
E Vuorio, and
Z Bösze, and
R Fässler, and
A Aszódi
July 2001,
Archives of biochemistry and biophysics,
