Biomaterial Database

Bioaugmentation with resin-acid-degrading bacteria enhances resin acid removal in sequencing batch reactors treating pulp mill effluents. 2001

Z Yu, and W W Mohn

Department of Microbiology and Immunology, and Pulp and Paper Centre, University of British Columbia, Vancouver, Canada.

Resin acids are the major toxicants in pulp and paper mill effluents (PPMEs), and they form pitch interfering with papermaking. Efficient and reliable resin acid removal is critically important to prevent toxicity discharge and ensure proper functioning of paper machines. Two resin-acid-degrading bacteria, Pseudomonas abietaniphila BKME-9 and Zoogloea resiniphila DhA-35, were tested in laboratory sequencing batch reactors (SBRs) for their ability to enhance resin acid removal by biomass from a full-scale biotreatment system treating PPMEs. Both bacteria enhanced resin acid removal but not removal of total organic carbon (TOC) by either pH-shocked or starved activated sludge. These two bacteria also increased resin acid removal when the sludge was given high concentration (200 microM) of resin acid. A most-probable-number polymerase chain reaction (MPN-PCR) assay showed that these two bacteria were initially not detectable (detection limit: 10(2) bacterial cells/ml) in the sludge community and were persistent after inoculation. Both bacteria did not substantially change the indigenous microbial community composition, as assayed by ribosomal intergenic spacer analysis (RISA). Our results suggest that it is feasible and potentially useful to enhance resin acid removal by bioaugmentation using resin-acid-degrading bacteria such as BKME-9 and DhA-35.

UI	MeSH Term	Description	Entries
D010209	Paper	Thin sheets made from wood pulp and other fibrous substances, used for writing, drawing, printing, image duplication or wrapping.	Papers
D011549	Pseudomonas	A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.	Chryseomonas,Pseudomona,Flavimonas
D004224	Diterpenes	Twenty-carbon compounds derived from MEVALONIC ACID or deoxyxylulose phosphate.	Diterpene,Diterpenes, Cembrane,Diterpenes, Labdane,Diterpenoid,Labdane Diterpene,Norditerpene,Norditerpenes,Norditerpenoid,Cembranes,Diterpenoids,Labdanes,Norditerpenoids,Cembrane Diterpenes,Diterpene, Labdane,Labdane Diterpenes
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001673	Biodegradation, Environmental	Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.	Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D014865	Waste Disposal, Fluid	The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.	Disposal, Fluid Waste,Disposals, Fluid Waste,Fluid Waste Disposal,Fluid Waste Disposals,Waste Disposals, Fluid
D014874	Water Pollutants, Chemical	Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water.	Chemical Water Pollutants,Landfill Leachate,Leachate, Landfill,Pollutants, Chemical Water
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D045784	Abietanes	A subclass of cyclical DITERPENES that contain the abietane ring structure.	Abietane Diterpene,Abietane Diterpenoid,Abietane Diterpenoids,Diterpenes, Abietane,Isopimarane,Isopimaranes,Totarane Diterpene,Totarane Diterpenes,Totaranes,Diterpenes, Pimarane,Pimaranes,Abietane Diterpenes,Diterpene, Abietane,Diterpene, Totarane,Diterpenoid, Abietane,Pimarane Diterpenes