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Natural killer (NK) cells are an early source of immunoregulatory cytokines during the innate immune response to viruses, bacteria, and parasites. NK cells provide requisite IFN-gamma to monocytes for the elimination of obligate intracellular pathogens. IL-1beta is a pro-inflammatory cytokine produced by monocytes (i.e. a monokine) during the early immune response to infection, but its role in promoting human NK cell IFN-gamma production is unknown. The current study examines the ability of the monokine IL-1beta, plus IL-12, to costimulate IFN-gamma production by resting CD56(bright) and CD56(dim) human NK cell subsets. CD56(bright) NK cells stimulated with IL-1beta plus IL-12 produced abundant IFN-gamma protein, while little IFN-gamma was produced in identical cultures of CD56(dim) cells. In addition, upon activation with IL-1beta, CD56(bright) NK cells exhibited considerably greater phosphorylation of extracellular signal-regulated kinases p42/44 as compared to CD56(dim) NK cells. Quantitative PCR analysis showed brisk induction of IFN-gamma gene expression following costimulation with IL-1beta plus IL-12 in CD56(bright) NK cells, but intracellular flow cytometry revealed that only a fraction (42+/-2.3%) of CD56(bright) NK cells account for this high IFN-gamma production. These data suggest that the monokine IL-1beta is a potent costimulus of IFN-gamma production by a subset of NK cells following infectious insult.
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
August 1996,
Clinical and experimental immunology,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
January 2019,
iScience,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
April 1985,
Journal of immunology (Baltimore, Md. : 1950),
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
October 2001,
Molecular human reproduction,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
December 2004,
Mediators of inflammation,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
May 1999,
Transplantation proceedings,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
January 1989,
Natural immunity and cell growth regulation,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
December 1995,
The Journal of clinical investigation,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
February 1999,
Inflammation,
M A Cooper, and
T A Fehniger, and
A Ponnappan, and
V Mehta, and
M D Wewers, and
M A Caligiuri
February 1993,
Journal of interferon research,
