Biomaterial Database

Production of methionine-excreting mutants of Streptomyces fradiae. 1975

R A Young, and R E Smith

Methionine-excreting mutant strains of Streptomyces fradiae ATCC 10745, producedby exposure to N-methyl-N'-nitro-N-nitrosoguanidine, were selected on the basisof ethionine-resistance and ability to support the growth of an auxotroph which requiredmethionine or methionine precursors. When acid hydrolysates of dehydrated liquid cultures were analyzed for amino acid content, one of the mutants had produced 268% more methionine that the parental strain. To obtain an accurate analysis of methionine content, it was necessary to determine the amount of diaminopimelic acid which had been synthesized. This compound appeared in eluates during column chromatography at about the same time as methionine, and its presence increased apparent methionine concentrations by 25 to40%.

UI	MeSH Term	Description	Entries
D008715	Methionine	A sulfur-containing essential L-amino acid that is important in many body functions.	L-Methionine,Liquimeth,Methionine, L-Isomer,Pedameth,L-Isomer Methionine,Methionine, L Isomer
D008769	Methylnitronitrosoguanidine	A nitrosoguanidine derivative with potent mutagenic and carcinogenic properties.	Methylnitrosonitroguanidine,Nitrosomethylnitroguanidine,Nitrosonitromethylguanidine,MNNG,N-Methyl-N'-nitro-N-nitrosoguanidine,N Methyl N' nitro N nitrosoguanidine
D009153	Mutagens	Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.	Clastogen,Clastogens,Genotoxin,Genotoxins,Mutagen
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D009604	Nitrosoguanidines	Nitrosylated derivatives of guanidine. They are used as MUTAGENS in MOLECULAR BIOLOGY research.
D003960	Diaminopimelic Acid	A diamino derivative of heptanedioic acid with amino groups at C-2 and C-6 and the general formula (COOH)CH(NH2)CH2CH2CH2CH(NH2)(COOH).	2,6-Diaminopimelic Acid,2,6 Diaminopimelic Acid,Acid, 2,6-Diaminopimelic,Acid, Diaminopimelic
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.