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Neisseria cuniculi produces the restriction enzyme NcuI which is an isoschizomer of MboII. We have demonstrated that NcuI recognizes a pentanucleotide sequence (5'-GAAGA-3'/3'-CTTCT-5'), and cleaves the DNA 8 and 7 nucleotides downstream from the recognition site leaving a single 3'-protruding nucleotide. We have purified this enzyme to electrophoretic homogeneity using a four-step chromatographic procedure. NcuI endonuclease is a monomeric protein with a M(r)=48,000+/-1000 under denaturing conditions. The properties of NcuI are consistent with those for MboII, the position of the cleavage site being identical and the pH profile and divalent cation requirements being similar. Moreover, NcuI cross-reacts strongly with anti-MboII serum suggesting the presence of similar antigenic determinants. We have determined the sequence of 20 N-terminal amino acids for NcuI and concluded that this sequence is identical to the N-terminal portion of the MboII enzyme.
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
April 1988,
Nucleic acids research,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
May 1995,
Gene,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
January 2015,
PloS one,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
March 2007,
Research in microbiology,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
January 1987,
Prikladnaia biokhimiia i mikrobiologiia,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
August 2008,
Biochemistry,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
May 2023,
Nucleic acids research,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
February 1990,
FEMS microbiology letters,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
July 2010,
PloS one,
B Furmanek, and
K Gromek, and
M Sektas, and
T Kaczorowski
May 2009,
Nucleic acids research,
