Arabidopsis thaliana flavoprotein AtHAL3a catalyzes the decarboxylation of 4'-Phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis. 2001

T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, Auf der Morgenstelle 15, Verfügungsgebäude, 72076 Tübingen, Germany.

The Arabidopsis thaliana flavoprotein AtHAL3a is related to plant growth and salt and osmotic tolerance. AtHAL3a shows sequence homology to the bacterial flavoproteins EpiD and Dfp. EpiD, Dfp, and AtHAL3a are members of the homo-oligomeric flavin-containing Cys decarboxylase (HFCD) protein family. We demonstrate that AtHAL3a catalyzes the decarboxylation of (R)-4'-phospho-N-pantothenoylcysteine to 4'-phosphopantetheine. This key step in coenzyme A biosynthesis is catalyzed in bacteria by the Dfp proteins. Exchange of His-90 of AtHAL3a for Asn led to complete inactivation of the enzyme. Dfp and AtHAL3a are characterized by a shortened substrate binding clamp compared with EpiD. Exchange of the cysteine residue of the conserved ACGD motif of this binding clamp resulted in loss of (R)-4'-phospho-N-pantothenoylcysteine decarboxylase activity. Based on the crystal structures of EpiD H67N with bound substrate peptide and of AtHAL3a, we present a model for the binding of (R)-4'-phospho-N-pantothenoylcysteine to AtHAL3a.

UI MeSH Term Description Entries
D008956 Models, Chemical Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment. Chemical Models,Chemical Model,Model, Chemical
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009097 Multienzyme Complexes Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES. Complexes, Multienzyme
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010088 Oxidoreductases The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9) Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010204 Pantetheine An intermediate in the pathway of coenzyme A formation in mammalian liver and some microorganisms.
D010205 Pantothenic Acid A butyryl-beta-alanine that can also be viewed as pantoic acid complexed with BETA ALANINE. It is incorporated into COENZYME A and protects cells against peroxidative damage by increasing the level of GLUTATHIONE. Vitamin B 5,Calcium Pantothenate,Dexol,Vitamin B5,Zinc Pantothenate,B 5, Vitamin,B5, Vitamin,Pantothenate, Calcium,Pantothenate, Zinc
D010453 Peptide Synthases Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups. Peptide Synthetases,Acid-Amino-Acid Ligases,Acid Amino Acid Ligases,Ligases, Acid-Amino-Acid,Synthases, Peptide,Synthetases, Peptide
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant

Related Publications

T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
March 2002, Journal of bacteriology,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
March 2006, The Journal of biological chemistry,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
October 2015, Molecular plant,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
February 2008, The FEBS journal,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
December 2022, ACS chemical biology,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
June 1970, The Journal of biological chemistry,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
September 1980, Biochemical and biophysical research communications,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
May 2000, The Journal of biological chemistry,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
October 2015, Nature chemical biology,
T Kupke, and P Hernandez-Acosta, and S Steinbacher, and F A Culianez-Macia
July 2000, FEBS letters,
Copied contents to your clipboard!