BIOMAT Database Logo BIOMAT Database Logo

Ca(2+)-sensor region of IP(3) receptor controls intracellular Ca(2+) signaling. 2001

T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, CREST, Japan.

Many important cell functions are controlled by Ca(2+) release from intracellular stores via the inositol 1,4,5-trisphosphate receptor (IP(3)R), which requires both IP(3) and Ca(2+) for its activity. Due to the Ca(2+) requirement, the IP(3)R and the cytoplasmic Ca(2+) concentration form a positive feedback loop, which has been assumed to confer regenerativity on the IP(3)-induced Ca(2+) release and to play an important role in the generation of spatiotemporal patterns of Ca(2+) signals such as Ca(2+) waves and oscillations. Here we show that glutamate 2100 of rat type 1 IP(3)R (IP(3)R1) is a key residue for the Ca(2+) requirement. Substitution of this residue by aspartate (E2100D) results in a 10-fold decrease in the Ca(2+) sensitivity without other effects on the properties of the IP(3)R1. Agonist-induced Ca(2+) responses are greatly diminished in cells expressing the E2100D mutant IP(3)R1, particularly the rate of rise of initial Ca(2+) spike is markedly reduced and the subsequent Ca(2+) oscillations are abolished. These results demonstrate that the Ca(2+) sensitivity of the IP(3)R is functionally indispensable for the determination of Ca(2+) signaling patterns.

UI MeSH Term Description Entries
D007424 Intracellular Fluid The fluid inside CELLS. Fluid, Intracellular,Fluids, Intracellular,Intracellular Fluids
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining

Related Publications

T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Cytosolic Ca(2+) controls the loading dependence of IP(3)-induced Ca(2+) release.
November 1999, Biochemical and biophysical research communications,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Ca(2+) signalling by IP(3) receptors.
January 2012, Sub-cellular biochemistry,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
IP(3) receptor function and localization in myotubes: an unexplored Ca(2+) signaling pathway in skeletal muscle.
October 2001, Journal of cell science,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Developmental aspects of cardiac Ca(2+) signaling: interplay between RyR- and IP(3)R-gated Ca(2+) stores.
June 2010, American journal of physiology. Heart and circulatory physiology,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
IP(3)-mediated Ca(2+) signals in human neuroblastoma SH-SY5Y cells with exogenous overexpression of type 3 IP(3) receptor.
August 2002, Cell calcium,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Agonist-evoked Ca(2+) wave progression requires Ca(2+) and IP(3).
August 2010, Journal of cellular physiology,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
IP(3) Receptors, Mitochondria, and Ca Signaling: Implications for Aging.
March 2011, Journal of aging research,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Stretch-induced Ca(2+) release via an IP(3)-insensitive Ca(2+) channel.
August 2002, American journal of physiology. Cell physiology,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Histamine potentiates IP(3)-mediated Ca(2+) release via thapsigargin-sensitive Ca(2+) pumps.
July 2003, Cellular signalling,
T Miyakawa, and A Mizushima, and K Hirose, and T Yamazawa, and I Bezprozvanny, and T Kurosaki, and M Iino
Hypotonic swelling-induced Ca(2+) release by an IP(3)-insensitive Ca(2+) store.
August 2001, American journal of physiology. Cell physiology,
Copied contents to your clipboard!