Biomaterial Database

[The influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation]. 2000

M Wasiela, and E Brzezińska-Błaszczyk

Zakład Mikrobiologii Lekarskiej A M w łodzi.

Nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. The aim of our study was to examine whether vaginal bacteria, especially those participating in Bacterial vaginosis, are able to activate mast cells to mediators secretion. The study was done on rat peritoneal mast cells. The mast cells were incubated in vitro with suspensions of Bacteroides capillosus, Actinomyces naeslundii (2 strains), Peptostreptococcus spp., Lactobacillus fermentum (2 strains), Mycoplasma hominis or Ureaplasma urealyticum killed by temperature. Activation of mast cells was estimated on the basis of histamine release. It was established that M. hominis, U. urealyticum and B. capillosus strongly stimulated rat mast cells to histamine secretion (histamine release 53.0%, 17.4% and 10.0%, respectively). Histamine release induced by Peptostreptococcus spp., A. naeslundii and L. fermentum was lower (at a range of 2.4%-8.2%). The obtained results can suggest that presumably interactions between vaginal bacteria and placental mast cells could influence the course of pregnancy.

UI	MeSH Term	Description	Entries
D008407	Mast Cells	Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.	Basophils, Tissue,Basophil, Tissue,Cell, Mast,Cells, Mast,Mast Cell,Tissue Basophil,Tissue Basophils
D009174	Mycoplasma	A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.	Eperythrozoon,Haemobartonella,Mycoplasma putrefaciens,PPLO,Pleuropneumonia-Like Organisms,Pleuropneumonia Like Organisms
D010537	Peritoneum	A membrane of squamous EPITHELIAL CELLS, the mesothelial cells, covered by apical MICROVILLI that allow rapid absorption of fluid and particles in the PERITONEAL CAVITY. The peritoneum is divided into parietal and visceral components. The parietal peritoneum covers the inside of the ABDOMINAL WALL. The visceral peritoneum covers the intraperitoneal organs. The double-layered peritoneum forms the MESENTERY that suspends these organs from the abdominal wall.	Parietal Peritoneum,Peritoneum, Parietal,Peritoneum, Visceral,Visceral Peritoneum,Parametrium,Parametriums
D005260	Female		Females
D006632	Histamine	An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.	Ceplene,Histamine Dihydrochloride,Histamine Hydrochloride,Peremin
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001421	Bacteria, Anaerobic	Bacteria that can survive and grow in the complete, or nearly complete absence of oxygen.	Anaerobic Bacteria
D014621	Vagina	The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)	Vaginas
D016585	Vaginosis, Bacterial	Polymicrobial, nonspecific vaginitis associated with positive cultures of Gardnerella vaginalis and other anaerobic organisms and a decrease in lactobacilli. It remains unclear whether the initial pathogenic event is caused by the growth of anaerobes or a primary decrease in lactobacilli.	Bacterial Vaginosis,Vaginitis, Bacterial,Vaginitis, Nonspecific,Bacterial Vaginitides,Bacterial Vaginitis,Bacterial Vaginoses,Vaginitides, Bacterial,Vaginoses, Bacterial,Nonspecific Vaginitis