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The activation of the nickel metalloenzyme urease is a complex process. In bacteria, several urease accessory proteins are essential for incorporation of nickel into the active centre of urease. Comparatively little is known about the activation process and the proteins involved in plants. We cloned five different cDNAs encoding isoforms of urease accessory protein G (ureG) in potato. The 5'-coding region of these cDNAs is highly polymorphic within Solanum tuberosum ssp. tuberosum, containing mainly a simple sequence repeat encoding histidine and aspartate. Mapping on an ultrahigh-density map of the potato genome and Southern blot analysis showed that the isoforms arise from allelic differences of a single-copy gene which was located on chromosome 2. Expression analysis at the mRNA and protein levels indicated the presence of ureG in almost all tissues examined, consistent with the ubiquitous expression of urease. An attempt to correlate urease activity with ureG expression levels in different tissues was made. Allelic copies of ureG were expressed in a tissue-specific manner. UreG from potato and the Klebsiella aerogenes urease operon defective in bacterial ureG were co-expressed in Escherichia coli. The plant gene complements the K. aerogenes ureG mutation, demonstrating that it encodes a urease accessory protein and indicating a structural conservation between the plant and the bacterial urease activation complexes.
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
July 1993,
Gene,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
September 2017,
The Journal of biological chemistry,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
October 2012,
Biochimica et biophysica acta,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
August 2006,
Acta biochimica et biophysica Sinica,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
October 2013,
PLoS biology,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
September 2014,
Biochimica et biophysica acta,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
March 2012,
Plant molecular biology,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
September 1999,
Proceedings of the National Academy of Sciences of the United States of America,
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
September 2018,
Methods (San Diego, Calif.),
C P Witte, and
E Isidore, and
S A Tiller, and
H V Davies, and
M A Taylor
July 2009,
The Biochemical journal,
