Zinc iodide-osmium tetroxide (ZIO) reactive sites in the extrafloral nectary of Citharexylum mirianthum Cham. (Verbenaceae). 2001

S R Machado, and E A Gregório
Departamento de botânica, Instituto de Biociências, UNESP, Botucatu, SP, Brazil. botanica@ibb.unesp.br

This paper reports on a study of the zinc iodide-osmium tetroxide method (ZIO) applicability to formaldehyde-glutaraldehyde prefixed extrafloral nectary tissues of Citharexylum mirianthum Cham. (Verbenaceae). The ZIO solution impregnates the dictyosome stacks and adjacent vesicles, smooth endoplasmic reticulum, nuclear envelope, multivesicular bodies, and peroxisomes. The use of this method greatly facilitates the observation and recognition of organelles in each nectary region. It also allows the correlation between structure and function in nectariferous cells.

UI MeSH Term Description Entries
D009993 Osmium Tetroxide (T-4)-Osmium oxide (OsO4). A highly toxic and volatile oxide of osmium used in industry as an oxidizing agent. It is also used as a histological fixative and stain and as a synovectomy agent in arthritic joints. Its vapor can cause eye, skin, and lung damage. Osmic Acid,Acid, Osmic,Tetroxide, Osmium
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D015388 Organelles Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES. Organelle
D017967 Zinc Compounds Inorganic compounds that contain zinc as an integral part of the molecule. Compounds, Zinc
D018515 Plant Leaves Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed) Plant Leaf,Leaf, Plant,Leave, Plant,Leaves, Plant,Plant Leave
D019441 Plant Epidermis A thin layer of cells forming the outer integument of seed plants and ferns. (Random House Unabridged Dictionary, 2d ed) Epidermis, Plant,Epidermi, Plant,Plant Epidermi
D019684 Magnoliopsida A class of vascular plants which produce flowers and seeds. They include monocotyledons, dicotyledons, and about 80% of all known plant species. Angiosperms,Angiosperma,Angiospermae,Arecidae,Asteridae,Caryophyllidae,Commelinidae,Dicotyledoneae,Dilleniidae,Flowering Plants,Hamamelidae,Hamamelididae,Icacinales,Liliatae,Liliidae,Liliopsida,Magnoliatae,Metteniusales,Oncothecales,Rosidae,Vahliales,Zingiberidae,Angiosperm,Flowering Plant,Icacinale,Metteniusale,Oncothecale,Plant, Flowering,Plants, Flowering,Vahliale

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