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Two high frequency antigens, Hy (Holley) and Gy-a (Gregory), show an apparent association. All individuals lacking Gy-a are whites and these individuals are Hy-negative. A second group of Hy-negative individuals are blacks. All of these react weakly with anti-Gy-a. Neither Gy-a nor Hy are well developed on cord cells, although antibodies to both factors have been stimulated by pregnancy. Anti-Gy-a and anti-Hy antibodies react best in the antihuman globulin test. These antibodies have low avidity, high titers, and have been implicated as causing transfusion reactions.
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