-transparent.png){kind=logo}
Hepatic UDP-glucuronyltransferase activity was resolved into two fractions, one exhibiting oestrone glucuronyltransferase activity and the other exhibiting p-nitrophenol glucuronyltransferase activity. Hydroxyapatite-column chromatography removed greater than 95% of the phospholipids from both preparations. The partially purified delipidated enzymes were essentially devoid of catalytic activity, but activities were restored by the addition of phospholipids or phosphatidylcholine mixtures containing various saturated and unsaturated fatty acids. Both oestrone and p-nitrophenol glucuronyl-transferase activities were reconstituted to similar degrees with the phosphatidylcholine mixtures. When purified phospholipids were tested, phosphatidylcholine and lysophosphatidylcholine were most effective in restoring activity, whereas phosphatidylethanolamine was the least effective. These results further suggest that oestrone and p-nitrophenol UDP-glucuronyltransferases are dependent on phospholipids for their activity.
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
February 1978,
Biochimica et biophysica acta,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
June 1973,
FEBS letters,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
November 1969,
Biochemical and biophysical research communications,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
January 1980,
Life sciences,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
October 1990,
Canadian journal of physiology and pharmacology,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
July 1982,
Biochemical and biophysical research communications,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
January 1970,
Enzymologia biologica et clinica,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
August 1980,
The Biochemical journal,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
January 1974,
Zeitschrift fur klinische Chemie und klinische Biochemie,
R H Tukey, and
R E Billings, and
A P Autor, and
T R Tephly
January 1979,
Enzyme,
