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The new generation of field emission in lens scanning electron microscopy with the sample located within the objective lens allows the observation of uncoated biological samples. Here we describe HeLa metaphase chromosomes isolated by two procedures and the localization within these chromosomes of a DNA centromeric probe. Chromosomes isolated in methanol acetic acid show 10 nm fibers organized in a three dimensional network while the structure of chromosomes obtained by the polyamine method's characterized by very dense short protusions of 30-50 nm in thickness. In situ hybridization with a DNA centromeric probe revealed by colloidal gold particles demonstrate that the hybridization occurs at the level of the 10 nm fiber with a high definition in the localization of specific DNA sequences.
G Mazzotti, and
L Cocco, and
F A Manzoli
August 1982,
Journal of cell science,
G Mazzotti, and
L Cocco, and
F A Manzoli
January 2004,
Methods in molecular biology (Clifton, N.J.),
G Mazzotti, and
L Cocco, and
F A Manzoli
April 1995,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
G Mazzotti, and
L Cocco, and
F A Manzoli
May 1996,
Cellular and molecular biology (Noisy-le-Grand, France),
G Mazzotti, and
L Cocco, and
F A Manzoli
January 1974,
Histochemistry,
G Mazzotti, and
L Cocco, and
F A Manzoli
October 1994,
Chromosoma,
G Mazzotti, and
L Cocco, and
F A Manzoli
January 1983,
Contributions to nephrology,
G Mazzotti, and
L Cocco, and
F A Manzoli
February 1997,
Microscopy research and technique,
G Mazzotti, and
L Cocco, and
F A Manzoli
June 2001,
Journal of microscopy,
G Mazzotti, and
L Cocco, and
F A Manzoli
January 1974,
Histochemistry,
