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A simple, rapid, and sensitive radioimmunoassay was developed for the determination of chlordiazepoxide (7-chloro-2-methylamino-5-phenyl-3H-1,4-benzodiazepine 4-oxide) in plasma. Antiserum capable of binding chlordiazepoxide-2-14C was obtained from rabbits following immunization with an antigen prepared by coupling the reactive acyl azide of 7-chloro-5-(4-hydrazinocarbonylmethoxyphenyl)-2-methylamino-3H-1,4-benzodiazepine 4-oxide to bovine serum albumin. The radioimmunoassay allows for the specific determination of chlordiazepoxide directly in plasma without extraction and was compared with a differential spectrofluorometric assay for chlordiazepoxide. Both methods gave satisfactory agreement for the plasma levels of chlordiazepoxide in human subjects resulting from single and chronic oral doses of chlordiazepoxide hydrochloride.
W R Dixon, and
J Earley, and
E Postma
February 1980,
Research communications in chemical pathology and pharmacology,
W R Dixon, and
J Earley, and
E Postma
June 1972,
Clinical chemistry,
W R Dixon, and
J Earley, and
E Postma
April 1977,
Scandinavian journal of clinical and laboratory investigation,
W R Dixon, and
J Earley, and
E Postma
September 1976,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
W R Dixon, and
J Earley, and
E Postma
November 1977,
The Journal of pharmacology and experimental therapeutics,
W R Dixon, and
J Earley, and
E Postma
December 1968,
The Journal of clinical investigation,
W R Dixon, and
J Earley, and
E Postma
August 1988,
Clinical chemistry,
W R Dixon, and
J Earley, and
E Postma
January 1972,
Comprehensive psychiatry,
W R Dixon, and
J Earley, and
E Postma
May 1979,
Journal of pharmaceutical sciences,
W R Dixon, and
J Earley, and
E Postma
January 1974,
The Journal of nuclear biology and medicine,
