BIOMAT Database Logo BIOMAT Database Logo

Lactoferrin regulates proliferative response of human peripheral blood mononuclear cells to phytohemagglutinin and mixed lymphocyte reaction. 2001

M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla, Wrocław, Poland. zimecki@immuno.iitd.pan.wroc.pl

The aim of this study was to investigate the effects of lactoferrin (LF) on the proliferative response of human peripheral blood mononuclear cells (PBMC) induced by phytohemagglutinin (PHA) and alloantigens in a two-way mixed lymphocyte reaction (MLR). The proliferative responses were measured by MTT colorimetric assay and presented as a proliferation index (PI) or as changes in PI caused by LF relative to PHA or MLR controls. We found that the effects of LF in both experimental models were differential and dependent on an individual PBMC reactivity, mitogen or alloantigen and LF concentration. Generally, lymphocytes from donors responsive to LF exhibited higher proliferation indices to PHA when compared with non-responsive individuals. Lactoferrin at low doses showed regulatory effects, whereas at higher doses the proliferation was significantly reduced. Mixed lymphocyte reaction was generally inhibited by LF. The results suggest that the differential action of LF might be due to its ability to sense the activation status of lymphocytes.

UI MeSH Term Description Entries
D007519 Isoantigens Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS. Alloantigens,Alloantigen,Isoantigen
D007781 Lactoferrin An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion. Lactotransferrin
D007959 Lymphocyte Culture Test, Mixed Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens. Leukocyte Culture Test, Mixed,Mixed Lymphocyte Culture Test,Mixed Lymphocyte Reaction,Mixed Leukocyte Culture Test,Mixed Leukocyte Reaction,Leukocyte Reaction, Mixed,Leukocyte Reactions, Mixed,Lymphocyte Reaction, Mixed,Lymphocyte Reactions, Mixed,Mixed Leukocyte Reactions,Mixed Lymphocyte Reactions
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D010835 Phytohemagglutinins Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture. Kidney Bean Lectin,Kidney Bean Lectins,Lectins, Kidney Bean,Phaseolus vulgaris Lectin,Phaseolus vulgaris Lectins,Phytohemagglutinin,Hemagglutinins, Plant,Lectin, Kidney Bean,Lectin, Phaseolus vulgaris,Lectins, Phaseolus vulgaris,Plant Hemagglutinins
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M

Related Publications

M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Does an autologous mixed lymphocyte reaction account for the "spontaneous" proliferative activity of peripheral blood mononuclear cells?
March 1982, Cellular immunology,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Effect of menstrual blood-derived stromal stem cells on proliferative capacity of peripheral blood mononuclear cells in allogeneic mixed lymphocyte reaction.
May 2012, The journal of obstetrics and gynaecology research,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Proliferative response of human peripheral blood mononuclear cells to Japanese encephalitis virus.
January 1995, Microbiology and immunology,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
In vitro proliferative response of human peripheral blood mononuclear cells to concanavalin A.
August 1997, Clinica chimica acta; international journal of clinical chemistry,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
In vitro response of peripheral blood mononuclear cells to phytohemagglutinin and interleukin-2.
March 1991, Human immunology,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Exposure of human peripheral blood mononuclear cells (PBMC) to hydrostatic pressure increases their proliferative response to phytohemagglutinin A (PHA) and anti-CD3 antibody.
January 1999, Immunological investigations,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
The human T-cell proliferative response to triple-knockout pig cells in mixed lymphocyte reaction.
September 2020, Xenotransplantation,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Enhanced proliferative response of myasthenic thymus cells in mixed lymphocyte reaction.
October 1992, Journal of the neurological sciences,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Aliphatic aldehydes inhibit the proliferative response of human peripheral blood lymphocytes to phytohemagglutinin and alloantigens.
January 1987, Chemico-biological interactions,
M Zimecki, and D Stepniak, and A Szynol, and M L Kruzel
Effect of asbestos exposure on differentiation of cytotoxic T lymphocytes in mixed lymphocyte reaction of human peripheral blood mononuclear cells.
July 2013, American journal of respiratory cell and molecular biology,
Copied contents to your clipboard!