BIOMAT Database Logo BIOMAT Database Logo

Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice. 2001

S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune response. For this purpose, a liquid N2 spray was applied on human melanoma (IIB-MEL-J cell line) xenografted in nude mice and 24 h later some mice received intratumorally a single 500 U dose of recombinant murine granulocyte macrophage-colony-stimulating factor. 77-100% of the tumor mass underwent necrosis. Congestion, edema, and endothelial cell activation were the first noticeable events. A quick infiltrative response of polymorphonuclear leukocytes around the tumor was detected 24 h after liquid N2 application, peaking at day 3. Massive macrophage recruitment was observed since day 3. An early intratumoral infiltration with inflammatory cells was only detected in the group that received recombinant murine granulocyte macrophage- colony-stimulating factor after necrosis induction by liquid N2. Coexisting DEC 205- and F4/80-positive cells increased in number, and their localization was predominantly peritumoral after necrosis. Antibody response was only detected in the groups with tumor-induced necrosis. Our results suggest that cryosurgery-induced necrosis could be a useful model to analyze the interaction among necrosis, inflammation, and the generation of an immune response.

UI MeSH Term Description Entries
D007249 Inflammation A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function. Innate Inflammatory Response,Inflammations,Inflammatory Response, Innate,Innate Inflammatory Responses
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D008545 Melanoma A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445) Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D009336 Necrosis The death of cells in an organ or tissue due to disease, injury or failure of the blood supply.
D009368 Neoplasm Transplantation Experimental transplantation of neoplasms in laboratory animals for research purposes. Transplantation, Neoplasm,Neoplasm Transplantations,Transplantations, Neoplasm
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D011184 Postoperative Period The period following a surgical operation. Period, Postoperative,Periods, Postoperative,Postoperative Periods
D003452 Cryosurgery The use of freezing as a special surgical technique to destroy or excise tissue. Cryoablation,Cryoablations,Cryosurgeries
D004487 Edema Abnormal fluid accumulation in TISSUES or body cavities. Most cases of edema are present under the SKIN in SUBCUTANEOUS TISSUE. Dropsy,Hydrops,Anasarca

Related Publications

S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Progression markers in metastasizing human melanoma cells xenografted to nude mice (review).
January 1989, Anticancer research,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Chromosome findings in human neuroblastomas xenografted in nude mice.
May 1985, Japanese journal of cancer research : Gann,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Repigmentation of human skin xenografted onto nude mice.
May 1990, Archives of dermatology,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice.
January 1991, Clinical & experimental metastasis,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Vascular permeability factor expression influences tumor angiogenesis in human melanoma lines xenografted to nude mice.
January 1995, The American journal of pathology,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Drug- and radiation-induced resistance in a human neurogenic sarcoma xenografted in nude mice.
January 1993, Cancer chemotherapy and pharmacology,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
[Growth rate of human colorectal cancer xenografted in nude mice].
February 1987, Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Radiolocalization of xenografted human malignant melanoma by a monoclonal antibody (9.2.27) to a melanoma-associated antigen in nude mice.
September 1985, Cancer research,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Effect of cholecystokinin on human cholangiocarcinoma xenografted into nude mice.
March 1985, Cancer research,
S Gazzaniga, and A Bravo, and S R Goldszmid, and F Maschi, and J Martinelli, and J Mordoh, and R Wainstok
Heterogeneity of xenografted osteosarcoma. A human sarcoma transplanted into nude mice.
October 1991, Acta orthopaedica Scandinavica,
Copied contents to your clipboard!