BIOMAT Database Logo BIOMAT Database Logo

The 5' boundary of the human apolipoprotein B chromatin domain in intestinal cells. 2001

T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Research Institute, Palo Alto Medical Foundation, Palo Alto, California 94301, USA.

The 5' boundary of the chromosomal domain of the human apolipoprotein B (apoB) gene in intestinal cells has been localized and characterized. It is composed of two kinds of boundary elements; the first, functional boundary is an insulator activity exhibited by a 1.8 kb DNA fragment located between -58 and -56 kb upstream of the human apoB promoter. In this region, an enhancer-blocking activity has been mapped to a CTCF binding site that is located upstream of two apoB intestinal enhancers (IEs), the 315 IE and the 485 IE. The CTCF site represents a boundary between two types of chromatin structure: an open, DNaseI-sensitive region 3' of the CTCF site containing the intestinal regulatory elements and a closed, DNaseI-resistant region 5' of the CTCF site. The 1.8 kb fragment harboring the CTCF site also insulated mini-white transgenes against position effects in Drosophila melanogaster. The second, structural boundary is represented by a nuclear matrix attachment region (MAR), situated about 3 kb 5' of the CTCF site. This MAR may represent the 5' anchorage site for a chromosomal loop that functions to bring the intestinal regulatory elements closer to the apoB promoter.

UI MeSH Term Description Entries
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D002843 Chromatin The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. Chromatins
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004331 Drosophila melanogaster A species of fruit fly frequently used in genetics because of the large size of its chromosomes. D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D004742 Enhancer Elements, Genetic Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter. Enhancer Elements,Enhancer Sequences,Element, Enhancer,Element, Genetic Enhancer,Elements, Enhancer,Elements, Genetic Enhancer,Enhancer Element,Enhancer Element, Genetic,Enhancer Sequence,Genetic Enhancer Element,Genetic Enhancer Elements,Sequence, Enhancer,Sequences, Enhancer
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000076246 CCCTC-Binding Factor A repressor protein with poly(ADP)-ribose binding activity that binds CHROMATIN and DNA; its structure consisting of 11 CYS2-HIS2 ZINC FINGERS allows it to recognize many different DNA target sites. It functions as a repressor by binding to INSULATOR ELEMENTS and preventing interaction between promoters and nearby enhancers and silencers. It plays a critical role in EPIGENETIC PROCESSES, including GENOMIC IMPRINTING. CTCF Protein,DNA-Binding Protein CTCF,CCCTC Binding Factor,CTCF, DNA-Binding Protein,DNA Binding Protein CTCF
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain.
January 1995, Molecular and cellular biology,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Localization of apolipoprotein B in intestinal epithelial cells.
September 1976, Science (New York, N.Y.),
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Human apolipoprotein B gene intestinal control region.
June 2001, Biochemistry,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Apolipoprotein B-100 is the major form of this apolipoprotein secreted by human intestinal Caco-2 cells.
October 1988, Biochemical and biophysical research communications,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Deciphering the regulatory logic of a chromatin domain boundary.
July 2022, Nature genetics,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Human small-intestinal apolipoprotein B-48 oligosaccharide chains.
February 1991, The Biochemical journal,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Chromatin domain boundary element search tool for Drosophila.
May 2012, Nucleic acids research,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Insulator: from chromatin domain boundary to gene regulation.
November 2001, Human genetics,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Computational characterization of chromatin domain boundary-associated genomic elements.
October 2017, Nucleic acids research,
T J Antes, and S J Namciu, and R E Fournier, and B Levy-Wilson
Apolipoprotein B-100: immunolocalization and synthesis in human intestinal mucosa.
November 1990, Journal of lipid research,
Copied contents to your clipboard!