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The bacteriophage Cre recombinase provides a powerful approach for tissue-specific gene inactivation. Using a Cre transgene driven by the common alpha subunit of glycoprotein hormones (alphaGSU-Cre), we have previously inactivated steroidogenic factor 1 (SF-1) in the anterior pituitary, causing hypogonadotropic hypogonadism with sexual infantilism, sterility, and severe gonadal hypoplasia. We now explore the molecular mechanisms underlying a hypomorphic gonadal phenotype in mice carrying two floxed SF-1 alleles (F/F) relative to mice carrying one recombined and one floxed allele (F/R). Because their Cre-mediated disruption of the locus encoding SF-1 was less efficient, alphaGSU-Cre, F/F mice retained some gonadotropin-expressing cells in the anterior pituitary, thereby stimulating some gonadal function. This novel in vivo model for exploring the effects of differing levels of gonadotropins on gonadal development highlights the need for careful genotype-phenotype comparisons in studies using Cre recombinase to produce tissue-specific knockouts.
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
March 2009,
Molecular and cellular endocrinology,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
June 2013,
Journal of toxicologic pathology,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
January 2010,
Slovenski veterinarski zbornik = Slovenian veterinary research,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
July 2004,
Molecular endocrinology (Baltimore, Md.),
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
February 2002,
Endocrinology,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
June 2008,
Molecular endocrinology (Baltimore, Md.),
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
April 2022,
Endocrinology,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
November 2004,
Endocrine research,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
September 1999,
Annales d'endocrinologie,
L Zhao, and
M Bakke, and
Y Krimkevich, and
L J Cushman, and
A F Parlow, and
S A Camper, and
K L Parker
March 2009,
Progress in neuro-psychopharmacology & biological psychiatry,
