Biomaterial Database

Detection of Giardia lamblia cysts in non-fixed long-term stored human feces by direct immunofluorescence assay. 2001

N Morimoto, and C Komatsu, and M Nishida, and T Sugiura

Department of Clinical Laboratory, Kochi Medical School of Hospital, Kochi 783-0043, Japan. morimoto@med.kochi-ms.ac.jp

Giardia lamblia cysts in fecal specimens are detected by conventional morphological methods. The direct immunofluorescence monoclonal antibody assay (DFA) is also applied to detect G. lamblia cysts in feces, but little is known about the usefulness of DFA in fecal specimens stored under various conditions. The aim of the present study was to evaluate the DFA for detection of G. lamblia cysts and to compare these results with the direct smear method in long-term storage of non-fixed fecal specimens. Fecal specimens with G. lamblia cysts were stored in a refrigerator at 5 degrees C(14 samples), a freezer at -20 degrees C (9 samples), or in 3.9% formalin-saline solution at room temperature (28 samples). G. lamblia cysts were detected by DFA in all stored specimens, while they were detected in only 56% of refrigerated and 93% of frozen specimens by the direct smear method. The storage period of all samples testing negative by direct smear was more than 24 months. Many degenerated cysts were recognized by DFA when cysts were negative by the direct smear method. Our results indicate that DFA is a useful method for detecting G. lamblia cysts in fecal samples that have undergone long-term storage.

UI	MeSH Term	Description	Entries
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005557	Formaldehyde	A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)	Formalin,Formol,Methanal,Oxomethane
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D005873	Giardiasis	An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA. It is spread via contaminated food and water and by direct person-to-person contact.	Lambliasis,Giardia Infection,Giardia duodenalis Infection,Giardia intestinalis Infection,Giardia lamblia Infection,Giardia Infections,Giardia duodenalis Infections,Giardia intestinalis Infections,Giardia lamblia Infections,Giardiases,Infection, Giardia,Infection, Giardia duodenalis,Infection, Giardia intestinalis,Infection, Giardia lamblia,Infections, Giardia,Infections, Giardia duodenalis,Infections, Giardia intestinalis,Infections, Giardia lamblia,Lambliases
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D016829	Giardia lamblia	A species of parasitic EUKARYOTES that attaches itself to the intestinal mucosa and feeds on mucous secretions. The organism is roughly pear-shaped and motility is somewhat erratic, with a slow oscillation about the long axis.	Giardia duodenalis,Giardia intestinalis,Lamblia intestinalis,Giardia lamblias
D019085	Fluorescent Antibody Technique, Direct	A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)	Immunofluorescence Antibody Test, Direct,Immunofluorescence Technique, Direct,Direct Fluorescent Antibody Technic,Direct Fluorescent Antibody Technique,Direct Immunofluorescence,Direct Immunofluorescence Assay,Fluorescent Antibody Technic, Direct,Immunofluorescence Technic, Direct,Assay, Direct Immunofluorescence,Assays, Direct Immunofluorescence,Direct Immunofluorescence Assays,Direct Immunofluorescence Technic,Direct Immunofluorescence Technics,Direct Immunofluorescence Technique,Direct Immunofluorescence Techniques,Direct Immunofluorescences,Immunofluorescence Assay, Direct,Immunofluorescence Assays, Direct,Immunofluorescence Technics, Direct,Immunofluorescence Techniques, Direct,Immunofluorescence, Direct,Immunofluorescences, Direct