BIOMAT Database Logo BIOMAT Database Logo

Identification of neuropeptide FF-related peptides in rodent spinal cord. 2001

E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Institut de Pharmacologie et de Biologie Structurale, C.N.R.S. UMR 5089, 205 route de Narbonne, Toulouse Cedex, 31077 France.

Peptides which should be generated from the neuropeptide FF (NPFF) precursor were identified in mouse and rat spinal cord, by using reverse phase high pressure liquid chromatography with radioimmunoassay and electrospray mass spectrometry detection. In both species, two octapeptides, NPFF (Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) and NPSF (Ser-Leu-Ala-Ala-Pro-Gln-Arg-Phe-amide) were identified but a longer peptide NPA-NPFF (Asn-Pro-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) was present at the highest concentration in rat spinal cord. In mouse, the homologous peptide, SPA-NPFF (Ser-Pro-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) was not detected. Both peptides NPFF and NPSF reverse morphine-induced analgesia in the tail flick test. Our data reveal species differences in the maturation of NPFF precursor.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009020 Morphine The principal alkaloid in opium and the prototype opiate analgesic and narcotic. Morphine has widespread effects in the central nervous system and on smooth muscle. Morphine Sulfate,Duramorph,MS Contin,Morphia,Morphine Chloride,Morphine Sulfate (2:1), Anhydrous,Morphine Sulfate (2:1), Pentahydrate,Oramorph SR,SDZ 202-250,SDZ202-250,Chloride, Morphine,Contin, MS,SDZ 202 250,SDZ 202250,SDZ202 250,SDZ202250,Sulfate, Morphine
D009842 Oligopeptides Peptides composed of between two and twelve amino acids. Oligopeptide
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass

Related Publications

E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry.
December 2002, FEBS letters,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Species differences in the localization of neuropeptide FF receptors in rodent and lagomorph brain and spinal cord.
January 1996, Peptides,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Neuropeptide FF in the rat spinal cord during carrageenan inflammation.
January 1997, Peptides,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Release of neuropeptide FF (FLFQPQRF-NH2) from rat spinal cord.
October 1992, Brain research,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Affinity of neuropeptide FF analogs to opioid receptors in the rat spinal cord.
January 1998, Peptides,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Functional characterization of a human receptor for neuropeptide FF and related peptides.
May 2001, British journal of pharmacology,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Origin and distribution of neuropeptide-FF-like immunoreactivity in the spinal cord of rats.
May 1991, The Journal of comparative neurology,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Activation of spinal neuropeptide FF and the neuropeptide FF receptor 2 during inflammatory hyperalgesia in rats.
January 2003, Neuroscience,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Central administration of neuropeptide FF and related peptides attenuate systemic morphine analgesia in mice.
April 2011, Protein and peptide letters,
E Bonnard, and O Burlet-Schiltz, and B Francés, and H Mazarguil, and B Monsarrat, and J M Zajac, and A Roussin
Presence of neuropeptide FF receptors on primary afferent fibres of the rat spinal cord.
September 1996, Neuroscience,
Copied contents to your clipboard!