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We have developed a simple method which can recover the highly purified macrophages or monocytes in suspension from mouse peritoneal exudate cells and human perpheral blood mononuclear cells. Plastic Petri dishes coated overnight with heat-inactivated fetal calf serum (FCS) selectively bind macrophages and monocytes. The adherent macrophages and monocytes are easily removed by incubation in phosphate-buffered saline containing 0.2% ethylenediamine tetraacetate (EDTA) and 5% FCS, and recovered as a cell suspension with greater than 95% purity. A small number of isolated cells can restore the mitogenic response to phytohemagglutinin (PHA-P) of macrophages-depleted lymphocytes and can lyse 51Cr-labeled target cells in an antibody-dependent cell-mediated cytotoxicity system. Thus, the method should be valuable for studies of various functions of macrophages and monocytes from different immune tissues of man and animals.
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
February 1922,
American journal of public health (New York, N.Y. : 1912),
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
December 1964,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
October 1949,
Journal of bacteriology,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
November 1967,
The Journal of protozoology,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
September 1966,
Public health reports (Washington, D.C. : 1896),
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
January 1979,
Antonie van Leeuwenhoek,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
July 1966,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
June 1995,
In vitro cellular & developmental biology. Animal,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
January 1979,
Laboratornoe delo,
K Kumagai, and
K Itoh, and
S Hinuma, and
M Tada
June 1968,
Applied microbiology,
