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The effects of various concentrations of fluoride on the mineralization of embryonic chich tibiae were studied in vitro. After 4 days incubation in medium with a physiological Ca x PO4 product (1.8 mM2), fluoride promoted a highly significant dose-related enhancement of bone mineralization. In experiments where the medium Ca x PO4 product was varied by changing either Ca or PO4 while keeping the other constant at 1.8 mM, fluoried at 7.5 muM or at 100 muM affected bone mineralization differbntly at low and high products. At medium Ca x PO4 products below 3 mM2, fluoride significantly enhanced bone mineral deposition compared to paired control bones, while at products above 5 mM2, fluoride significantly inhibited bone mineralization. This dual effect of fluoride was observed in both live bones and bones metabolically inhibited by 1 mM iodoacetic acid. Comparison of the effects of fluoride on the mineralization of live and dead bones suggested that the effects are dependent on the cellular control of Ca x PO4 product in the bone extracellular fluid. These findings support the hypothesis that fluoride may play an important role in governing the rate of mineralization in growing bones.
E P Hicks, and
W K Ramp
June 1975,
Calcified tissue research,
E P Hicks, and
W K Ramp
December 1977,
Calcified tissue research,
E P Hicks, and
W K Ramp
January 1972,
Developmental biology,
E P Hicks, and
W K Ramp
July 1981,
General and comparative endocrinology,
E P Hicks, and
W K Ramp
January 1983,
Experimental cell biology,
E P Hicks, and
W K Ramp
May 1980,
Poultry science,
E P Hicks, and
W K Ramp
April 1979,
Calcified tissue international,
E P Hicks, and
W K Ramp
January 1957,
Bollettino della Societa italiana di biologia sperimentale,
E P Hicks, and
W K Ramp
November 1983,
Biochemical pharmacology,
