Biomaterial Database

Spectrophotometric determination of sparfloxacin in pharmaceutical formulations using bromothymol blue. 2001

H R Marona, and E E Schapoval

Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP. 14801-902, SP, Araraquara, Brazil. maronah@fcfar.unesp.br

A visible light spectrophotometric method is described for the determination of sparfloxacin in tablets. The procedure is based on the complexation of bromothymol blue 0.5% and sparfloxacin to form a compound of yellow colour with maximum absorption at 385 nm. The Lambert-Beer law was obeyed in the concentration range of 2-12 mg/l. The present study describes a sensitive and accurate method for the determination of the concentration of sparfloxacin in tablets. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay.

UI	MeSH Term	Description	Entries
D001979	Bromthymol Blue	A pH sensitive dye that has been used as an indicator in many laboratory reactions.	Dibromothymolsulfonphthalein,Bromothymol Blue,Blue, Bromothymol,Blue, Bromthymol
D002138	Calibration	Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.	Calibrations
D000890	Anti-Infective Agents	Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.	Anti-Infective Agent,Anti-Microbial Agent,Antimicrobial Agent,Microbicide,Microbicides,Anti-Microbial Agents,Antiinfective Agents,Antimicrobial Agents,Agent, Anti-Infective,Agent, Anti-Microbial,Agent, Antimicrobial,Agents, Anti-Infective,Agents, Anti-Microbial,Agents, Antiinfective,Agents, Antimicrobial,Anti Infective Agent,Anti Infective Agents,Anti Microbial Agent,Anti Microbial Agents
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D024841	Fluoroquinolones	A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.	Fluoroquinolone