OBJECTIVE Lack of transplant material is a main problem in the repair and reconstruction of tissue or organ defects. To seek the succedaneum of transplants, we studied and used tissue-engineered growth hyaline cartilage. METHODS The articular hyaline cartilage obtained from newborn calf within 6 hours of sacrifice was enzymatically dissolved. The chondrocytes were then seeded onto the medical collagen membrane of guided tissue regeneration in a 24 well plate in RPMI1640 medium. One week later, the chondrocyte medical collagen membrane of guided tissue regeneration complex was implanted subcutaneously into the back of nude mice. Eight weeks after implantation, the animals were sacrificed. RESULTS The present study seems to demonstrate that the cartilage-like tissue was strong enough to be transferred after being implanted for 8 weeks. The cartilage-like tissue was proved to be tissue-engineered hyaline cartilage by HE stain. The chondrocytes could secrete chondroitin sulfate as proved by Lev-Spicer stain. CONCLUSIONS Tissue engineering technique was used to make tissue-engineered cartilage in vitro. It will provide a new transplant material for the repair of tissue or organ defects.