[Simple rapid and economical procedure for agarose electrophoretic separation of serum lipoprotein fractions]. 1975

A Menegozzo, and M T Guidi, and S De Cecchi, and U Lippi

A detailed description is given of a rapid, simple and economic technique for the electrophoretic migration on agarose gel of serum lipoproteins. The albuminated agarose is spread hot on pieces of normal radiological plates even if already used. The reservoir for the serum is obtained by putting in the agarose while still hot a series of small metal bars. The moulds are removed with a small magnet as soon as the gel is formed. Plates prepared for 6-7 lipidograms can comfortably be kept for as long as 20-30 days. For stored plates ready for use, the analytic procedure does not require more than 40-45 minutes. The results can be considered altogether satisfactory.

UI MeSH Term Description Entries
D008074 Lipoproteins Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes. Circulating Lipoproteins,Lipoprotein,Lipoproteins, Circulating
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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