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A new method of isolation of highly purified Cl. botulinum toxin of E type from the cultural fluid of strain 188 centrifugates was developed. The method allows to isolate the toxin both in a precursor and in activated forms with a yield of 10--15%. The method includes fractionation by ammonium sulfate, ultrafiltration and subsequent column chromatography on DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex A-50. The preparations were found homogeneous during polyacrylamide gel electrophoresis and immunoprecipitation in agar with antitoxic horse serum. The potential specific toxicity of the preparations is 1--1,2.10(7) DLM/mg of protein. The molecular weight of the toxin is about 160 000; the molar extinction coefficient is equal to 278 nm. The isoelectric point lies around pH 6.0. The highly purified Cl. botulinum toxin of E type was found stable upon storage.
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
November 1980,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
December 1990,
Japanese journal of medical science & biology,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
April 1975,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
January 1968,
Medycyna doswiadczalna i mikrobiologia,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
October 1968,
Biochimica et biophysica acta,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
July 1986,
Journal of general microbiology,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
March 1968,
Nihon saikingaku zasshi. Japanese journal of bacteriology,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
October 1954,
Canadian journal of microbiology,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
November 1947,
Journal of bacteriology,
G A Levdikova, and
L V Klimacheva, and
M V Ispolatovskaia, and
T I Bulatova, and
L I Anisimova
March 1967,
Applied microbiology,
