Simple organ cornea culture model for re-epithelialization after in vitro excimer laser ablation. 2001

R S Chuck, and A Behrens, and S R Wellik, and L H Liaw, and P M Sweet, and K E Osann, and P J McDonnell, and M W Berns
Department of Ophthalmology, University of California Irvine, Irvine, California 92697, USA. rschuck@uci.edu

OBJECTIVE Most of the in vitro work to characterize the effects of clinical laser surgery on corneal tissues has concentrated on the effects on stromal keratocytes and endothelium with little attention being paid to corneal epithelium. Our purpose is to describe the epithelial healing rates observed in freshly cultured rabbit corneas treated with phototherapeutic keratectomy (PTK). METHODS Corneas were placed in a simple organ culture system, with media change every 2 days. A clinical excimer laser was used to perform a 6 mm diameter, 100 microm depth transepithelial PTK on 24 cultured rabbit corneas, 1 day after culture initiation. For each post-treatment day, one experimental and one control cornea were removed from culture and stained with fluorescein, photographed, and fixed for histology. Epithelial defect area was measured with digital imaging software and analyzed statistically to assess the re-epithelialization rate. RESULTS Control corneas, maintained in culture for 1-4 days, had no epithelial defects. Those corneas treated with PTK exhibited an immediate epithelial defect that slowly healed over 3 days. This was confirmed on histopathological analysis. A significant linear trend in re-epithelialization across the time points studied was found (F = 80.48, P = 0.0029). The slope of the linear regression model showed an estimate rate of re-epithelialization of -6.70 over the 3 days. CONCLUSIONS We have described the development of a simple, whole organ, rabbit cornea culture model for re-epithelialization after PTK. Our rates of epithelial healing resemble those found in the literature in live rabbit models. Therefore, this model may possibly be used to monitor epithelial wound healing in different corneal diseases or injuries.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009924 Organ Culture Techniques A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1) Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011833 Radiation Injuries, Experimental Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals. Experimental Radiation Injuries,Injuries, Experimental Radiation,Experimental Radiation Injury,Radiation Injury, Experimental
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014945 Wound Healing Restoration of integrity to traumatized tissue. Healing, Wound,Healings, Wound,Wound Healings
D054018 Lasers, Excimer Gas lasers with excited dimers (i.e., excimers) as the active medium. The most commonly used are rare gas monohalides (e.g., argon fluoride, xenon chloride). Their principal emission wavelengths are in the ultraviolet range and depend on the monohalide used (e.g., 193 nm for ArF, 308 nm for Xe Cl). These lasers are operated in pulsed and Q-switched modes and used in photoablative decomposition involving actual removal of tissue. (UMDNS, 2005) Argon Fluoride Excimer Lasers,Excimer Lasers,Krypton Chloride Excimer Lasers,Xenon Chloride Excimer Lasers,ArF Excimer Lasers,ArF Lasers,Argon Fluoride Lasers,Excimer Laser,Excimer Lasers, ArF,Excimer Lasers, Argon Fluoride,Excimer Lasers, KrCl,Excimer Lasers, Krypton Chloride,Excimer Lasers, XeCl,Excimer Lasers, Xenon Chloride,KrCl Excimer Lasers,Krypton Chloride Lasers,Lasers, ArF Excimer,Lasers, Argon Fluoride Excimer,Lasers, KrCl,Lasers, Krypton Chloride,Lasers, Krypton Chloride Excimer,Lasers, XeCl Excimer,Lasers, Xenon Chloride,Lasers, Xenon Chloride Excimer,XeCl Lasers,Xenon Chloride Lasers,ArF Excimer Laser,ArF Laser,Argon Fluoride Laser,Chloride Laser, Krypton,Chloride Laser, Xenon,Chloride Lasers, Krypton,Chloride Lasers, Xenon,Excimer Laser, ArF,Excimer Laser, KrCl,Excimer Laser, XeCl,Fluoride Laser, Argon,Fluoride Lasers, Argon,KrCl Excimer Laser,KrCl Laser,KrCl Lasers,Krypton Chloride Laser,Laser, ArF,Laser, ArF Excimer,Laser, Argon Fluoride,Laser, Excimer,Laser, KrCl,Laser, KrCl Excimer,Laser, Krypton Chloride,Laser, XeCl,Laser, XeCl Excimer,Laser, Xenon Chloride,Lasers, ArF,Lasers, Argon Fluoride,Lasers, KrCl Excimer,Lasers, XeCl,XeCl Excimer Laser,XeCl Excimer Lasers,XeCl Laser,Xenon Chloride Laser
D018903 Photorefractive Keratectomy A type of refractive surgery of the CORNEA to correct MYOPIA and ASTIGMATISM. An EXCIMER LASER is used directly on the surface of the EYE to remove some of the CORNEAL EPITHELIUM thus reshaping the anterior curvature of the cornea. Keratectomies, Photorefractive,Keratectomy, Photorefractive,Photorefractive Keratectomies

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