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Fragments of liver from the adult urodele Amphiuma means, the Congo eel, were maintained in organ culture for up to 70 days. The normal electrophoretic patterns of several enzymes were retained. The activities of ornithine transcarbamylase, arginase, glutamate oxalacetate transaminase and glutamate pyruvate transaminase, and urea production, glucose uptake and tissue glycogen content remained relatively constant throughout the culture period. Histological organization and hepatocyte ultrastructure were also retained. Liver fragments survived better in media based on MEM or BME than in medium based on Leibovitz L15. Since many aspects of tissue-specific structure and function are retained, long-term amphibian organ culture is well suited to studies on the control of hepatocyte function and on the effects of metabolites, hormones, drugs and toxins.
N Fleming, and
D Brown, and
M Balls
February 1989,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
N Fleming, and
D Brown, and
M Balls
September 1977,
General and comparative endocrinology,
N Fleming, and
D Brown, and
M Balls
September 1977,
General and comparative endocrinology,
N Fleming, and
D Brown, and
M Balls
June 1992,
Experimental cell research,
N Fleming, and
D Brown, and
M Balls
January 1980,
Annals of the New York Academy of Sciences,
N Fleming, and
D Brown, and
M Balls
February 1974,
Comparative biochemistry and physiology. A, Comparative physiology,
N Fleming, and
D Brown, and
M Balls
February 1983,
Investigative ophthalmology & visual science,
N Fleming, and
D Brown, and
M Balls
January 1983,
Cancer research,
N Fleming, and
D Brown, and
M Balls
August 1977,
In vitro,
N Fleming, and
D Brown, and
M Balls
March 1976,
Cancer research,
