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Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as parathyroid hormone and proparathyroid hormone. Based on thin layer gel filtration in the presence of 6 M guanidine-HCl, the proparathyroid hormone had a molecular weight of 11,500 compared to about 9600 for parathyroid hormone. Radioisotope sequence analysis of the proparathyroid hormone revealed a partial sequence of: Lys1-Pro2-Ile3-Lys4-Lys5-Arg6-Ser7-Val8-Ser9--Ile11--Met14--Gly18--Ser22--Ser23---. Thus, from position 7 onward the relative position of each amino acid tested in this molecule corresponded exactly to that in the porcine parathyroid hormone sequence. The conservation of a similar, though not identical, basic hexapeptide grouping Lys-X-Y-Lys-Lys-Arg- at the amino terminal region of the prohormone in all species examined thus far (porcine, human, and bovine) suggests that this segment of the molecule may play an important role in the conversion of the prohormone to the hormone.
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E T Littledike, and
J W Hamilton, and
D V Cohn
March 1974,
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L K Chu, and
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D V Cohn
January 1976,
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L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
July 1970,
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L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
January 1981,
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L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
April 1974,
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L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
January 1970,
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae,
L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
January 1971,
Biochimica et biophysica acta,
L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
June 1978,
Proceedings of the National Academy of Sciences of the United States of America,
L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
September 1979,
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L K Chu, and
W Y Huang, and
E T Littledike, and
J W Hamilton, and
D V Cohn
November 1972,
Science (New York, N.Y.),
