BIOMAT Database Logo BIOMAT Database Logo

Molecular characterization of a new abortive infection system (AbiU) from Lactococcus lactis LL51-1. 2001

G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Department of Biotechnology, The University of New South Wales, Sydney, New South Wales 2052, Australia.

This study reports on the identification and characterization of a novel abortive infection system, AbiU, from Lactococcus lactis. AbiU confers resistance to phages from the three main industrially relevant lactococcal phage species: c2, 936, and P335. The presence of AbiU reduced the efficiency of plaquing against specific phage from each species as follows: 3.7 x 10(-1), 1.0 x 10(-2), and 1.0 x 10(-1), respectively. abiU involves two open reading frames, abiU1 (1,772 bp) and abiU2 (1,019 bp). Evidence indicates that AbiU1 is responsible for phage resistance and that AbiU2 may downregulate phage resistance against 936 and P335 type phages but not c2 type phage. AbiU appeared to delay transcription of both phage 712 and c2, with the effect being more marked on phage c2.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013294 Lactococcus lactis A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID. Streptococcus lactis,Lactococcus lactis subsp. lactis
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription

Related Publications

G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
AbiQ, an abortive infection mechanism from Lactococcus lactis.
December 1998, Applied and environmental microbiology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
A novel plasmid-encoded phage abortive infection system from Lactococcus lactis biovar. diacetylactis.
January 1997, FEMS microbiology letters,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Characterization of the two-component abortive phage infection mechanism AbiT from Lactococcus lactis.
November 2002, Journal of bacteriology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Phenotypic and genetic characterization of the bacteriophage abortive infection mechanism AbiK from Lactococcus lactis.
April 1997, Applied and environmental microbiology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Molecular characterization of a second abortive phage resistance gene present in Lactococcus lactis subsp. lactis ME2.
November 1992, Journal of bacteriology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Genetic organization and functional analysis of a novel phage abortive infection system, AbiL, from Lactococcus lactis.
January 1999, Journal of biotechnology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Cloning and characterization of the abortive infection genetic determinant abiD isolated from pBF61 of Lactococcus lactis subsp. lactis KR5.
May 1995, Applied and environmental microbiology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Controlled Integration into the Lactococcus Chromosome of the pCI829-Encoded Abortive Infection Gene from Lactococcus lactis subsp. lactis UC811.
October 1992, Applied and environmental microbiology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2.
June 2000, Applied and environmental microbiology,
G Dai, and P Su, and G E Allison, and B L Geller, and P Zhu, and W S Kim, and N W Dunn
Molecular characterization of a phage-encoded resistance system in Lactococcus lactis.
May 1999, Applied and environmental microbiology,
Copied contents to your clipboard!