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Promyelocytic leukemia (PML) protein is involved in apoptotic death of cultured neuronal cells, but its role in ischemic brain damage remains uncertain. In this study, we investigated change of immunoreactivity for PML protein in rat brain after transient middle cerebral artery occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL). Western blotting analysis revealed that PML immunoreactivity was only scant in the sham-control brain, but it increased at 1 h and 1 day after reperfusion, and decreased in density thereafter. Immunohistochemical analysis revealed that nuclei of neurons were most densely stained. TUNEL positive cells appeared at 1 day and peaked at 3 days of reperfusion, indicating that PML protein induction preceded DNA fragmentation in neurons. The present results suggest that PML protein may be one of the key molecules in ischemic neuronal cell death.
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
July 1998,
Neurological research,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
June 1998,
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
November 2002,
Brain research,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
March 2005,
Brain research,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
January 1995,
Journal of the neurological sciences,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
August 2003,
Journal of neuroscience research,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
May 2008,
The Journal of pharmacology and experimental therapeutics,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
July 1996,
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
December 1999,
Journal of the neurological sciences,
T Hayashi, and
C Sasaki, and
M Iwai, and
K Sato, and
W R Zhang, and
H Warita, and
K Abe
January 1994,
Acta neuropathologica,
