Activation of chondrogenesis in response to injury in normal and transgenic mice with cartilage collagen mutations. 2001

H Uusitalo, and H Salminen, and E Vuorio
Department of Medical Biochemistry and Molecular Biology, University of Turku, Finland.

OBJECTIVE The aim of this report is to describe and discuss the reparative capacity of articular cartilage by focusing on similarities and differences in the activation of chondrogenesis in adult bone and cartilage in response to injury. METHODS The present report describes three different models of skeletal repair in the mouse. Two of the models deal with bone healing, where the activation of chondrogenesis and formation of callus tissue is greatly dependent on the rigidity of fixation. The third comprises two transgenic mouse models for osteoarthritis where dominant negative mutations in cartilage-specific genes disturb the structural integrity of the cartilage collagen fibrils. RESULTS Molecular biologic and immunohistochemical analyses demonstrated that activation of chondrogenesis in healing fractures, i.e., activation and maintenance of the chondrocyte phenotype was preceded by increased production and nuclear accumulation of transcription factor SOX9. A similar, albeit smaller, chondrogenic response was observed during healing of biomechanically stable metaphyseal bone defects. In degenerating articular cartilage of transgenic mice, however, the production of cartilage-specific collagen types and SOX9 was markedly reduced upon aging which probably explains why repair of cartilage defects was insufficient. CONCLUSIONS Understanding of the molecular mechanisms involved in successful and unsuccessful activation of chondrogenesis during skeletal repair, will provide information needed for enhancement of the chondrocytic response at sites of skeletal repair. Our data also demonstrates that specific effector molecules can be efficiently introduced into chondrocytes and their precursors by adenovirus-mediated gene transfer.

UI MeSH Term Description Entries
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010003 Osteoarthritis A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans. Arthritis, Degenerative,Osteoarthrosis,Osteoarthrosis Deformans,Arthroses,Arthrosis,Arthritides, Degenerative,Degenerative Arthritides,Degenerative Arthritis,Osteoarthritides,Osteoarthroses
D012038 Regeneration The physiological renewal, repair, or replacement of tissue. Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D001842 Bone and Bones A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principal cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX. Bone Tissue,Bone and Bone,Bone,Bones,Bones and Bone,Bones and Bone Tissue,Bony Apophyses,Bony Apophysis,Condyle,Apophyses, Bony,Apophysis, Bony,Bone Tissues,Condyles,Tissue, Bone,Tissues, Bone
D002358 Cartilage, Articular A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact. Articular Cartilage,Articular Cartilages,Cartilages, Articular
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D006609 High Mobility Group Proteins A family of low-molecular weight, non-histone proteins found in chromatin. HMG Proteins,Calf Thymus Chromatin Protein HMG,High Mobility Group Chromosomal Proteins
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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