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The presence of poly(A) polymerase(s) has been studied in a clone of the established hamster embryo fibroblast line (NIL), and in a subclone of this line transformed by an RNA tumour virus, hamster sarcoma virus, (NIL-HS VIRUS). The results suggest the existence of three distinct poly(A) polymerases, designated I, IIA and IIB, in dense cultures of NIL and NIL-HS virus cells. Forms IIA and IIB have also been found in exponentially growing NIL and NIL-HS virus cells. Poly(A) polymerase I has not been detected in growing NIL cells, while growing and resting NIL-HS virus contain comparable amounts of this enzyme. The poly(A) polymerases differ in chromatographic behaviour and in their requirements for divalent cations. They are highly specific for ATP and require the presence of a primer. Cellular RNA or poly(A), but not the oligoribonucleotide (Ap)4A, can be utilized as primers. The products of the reactions have been identified as poly(A) chains (20-50 nucleotides long) by alkaline degradation and by their resistance to pancreatic RNAase.
A Pellicer, and
J Salas, and
M L Salas
April 1971,
Cancer research,
A Pellicer, and
J Salas, and
M L Salas
January 1971,
Vestnik Akademii meditsinskikh nauk SSSR,
A Pellicer, and
J Salas, and
M L Salas
September 1968,
International journal of cancer,
A Pellicer, and
J Salas, and
M L Salas
November 1975,
The Journal of cell biology,
A Pellicer, and
J Salas, and
M L Salas
February 1974,
Experimental cell research,
A Pellicer, and
J Salas, and
M L Salas
January 1974,
Biochemistry,
A Pellicer, and
J Salas, and
M L Salas
March 1975,
The Biochemical journal,
A Pellicer, and
J Salas, and
M L Salas
May 1973,
The Journal of biological chemistry,
A Pellicer, and
J Salas, and
M L Salas
February 1994,
Proceedings of the National Academy of Sciences of the United States of America,
A Pellicer, and
J Salas, and
M L Salas
December 1968,
Journal of cell science,
