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Disruption of the adhC gene of Mycobacterium smegmatis mc(2)155, by standard gene replacement methods, revealed that there are two copies of this gene within a large duplication of the M. smegmatis mc(2)155 genome. M. smegmatis AdhC(+/-) and M. smegmatis AdhC(-/-) mutants were obtained when one or two adhC copies, respectively, were disrupted by homologous recombination. Southern blot analysis of DraI restriction digests of the DNA from these mutants and from wild-type M. smegmatis mc(2)155, resolved by PFGE, showed that the duplication size may be at least approximately 250 kb. The single and double knockout mutants were characterized and compared with the M. smegmatis wild-type. A growth disadvantage and a different morphology were associated with the loss of expression of one or both of the adhC copies, but both mutants were still acid-fast. Findings in this study indicate that the process of chromosomal duplication in M. smegmatis is ongoing and remains a potent source of genome dynamics. Hence, the M. smegmatis mc(2)155 genome might be larger than previously thought.
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
April 1997,
FEMS microbiology letters,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
November 2006,
Tuberculosis (Edinburgh, Scotland),
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
October 2012,
Fitoterapia,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
February 2011,
Current microbiology,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
May 2016,
International journal of molecular sciences,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
March 2007,
Research in microbiology,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
June 2005,
Microbiology (Reading, England),
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
February 2009,
Vaccine,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
August 2013,
European journal of medicinal chemistry,
A Galamba, and
K Soetaert, and
X M Wang, and
J De Bruyn, and
P Jacobs, and
J Content
January 2009,
Journal of molecular microbiology and biotechnology,
