BIOMAT Database Logo BIOMAT Database Logo

Leptin effect on endothelial nitric oxide is mediated through Akt-endothelial nitric oxide synthase phosphorylation pathway. 2002

Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
IRCCS Neuromed, Pozzilli (IS), Italy.

Recent evidence suggests that besides its action on the central nervous system, leptin can modulate vascular tone through local mechanisms involving nitric oxide (NO) release. In this study, using a fluorescent probe for direct determination of NO, we demonstrated both in endothelial cells and in vessels that leptin is able to stimulate NO release. The effect of leptin on NO is abolished by erbstatin A, a Ca(2+)-independent tyrosine kinase inhibitor, whereas it is not influenced by calcium removal or by other protein phosphorylation inhibitors, such as genistein (an ATP-dependent tyrosine-kinase inhibitor) or wortmannin and LY294002 (two different phosphatidylinositol [PI] 3-kinase inhibitors). Accordingly, leptin-induced vasorelaxation in aortic rings was abolished only by erbstatin A. Furthermore, immunoblotting studies revealed that leptin evokes Akt phosphorylation, with a comparable time course in both endothelial cells and vessels. Also in this experimental system, the effect of leptin was abolished by erbstatin A and not by other inhibitors. Finally, a considerable increase in endothelial NO synthase (eNOS) phosphorylation in Ser(1177) was found when vessels were treated with leptin. In conclusion, leptin induces NO production by activating a PI 3-kinase-independent Akt-eNOS phosphorylation pathway.

UI MeSH Term Description Entries
D009025 Morpholines Tetrahydro-1,4-Oxazines,Tetrahydro 1,4 Oxazines
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011505 Protein-Tyrosine Kinases Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors. Tyrosine Protein Kinase,Tyrosine-Specific Protein Kinase,Protein-Tyrosine Kinase,Tyrosine Kinase,Tyrosine Protein Kinases,Tyrosine-Specific Protein Kinases,Tyrosylprotein Kinase,Kinase, Protein-Tyrosine,Kinase, Tyrosine,Kinase, Tyrosine Protein,Kinase, Tyrosine-Specific Protein,Kinase, Tyrosylprotein,Kinases, Protein-Tyrosine,Kinases, Tyrosine Protein,Kinases, Tyrosine-Specific Protein,Protein Kinase, Tyrosine-Specific,Protein Kinases, Tyrosine,Protein Kinases, Tyrosine-Specific,Protein Tyrosine Kinase,Protein Tyrosine Kinases,Tyrosine Specific Protein Kinase,Tyrosine Specific Protein Kinases
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D011921 Rats, Inbred WKY A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR). Rats, Wistar Kyoto,Wistar Kyoto Rat,Rats, WKY,Inbred WKY Rat,Inbred WKY Rats,Kyoto Rat, Wistar,Rat, Inbred WKY,Rat, WKY,Rat, Wistar Kyoto,WKY Rat,WKY Rat, Inbred,WKY Rats,WKY Rats, Inbred,Wistar Kyoto Rats
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002867 Chromones 1,4-Benzopyrones,Chromone,1,4 Benzopyrones
D004791 Enzyme Inhibitors Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D006873 Hydroquinones Derivatives of hydroquinone (1,4-dihydrobenzene) made by reduction of BENZOQUINONES. Quinol,p-Dihydroxybenzenes,para-Dihydroxybenzenes,Quinols,p Dihydroxybenzenes,para Dihydroxybenzenes

Related Publications

Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Potent vasodilation effect of amurensin G is mediated through the phosphorylation of endothelial nitric oxide synthase.
December 2012, Biochemical pharmacology,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection.
March 2002, Proceedings of the National Academy of Sciences of the United States of America,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Pitavastatin at low dose activates endothelial nitric oxide synthase through PI3K-AKT pathway in endothelial cells.
March 2005, Life sciences,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells.
June 2008, International journal of cardiology,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Thrombin and histamine stimulate endothelial nitric-oxide synthase phosphorylation at Ser1177 via an AMPK mediated pathway independent of PI3K-Akt.
August 2004, FEBS letters,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation.
June 1999, Nature,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
β-Amyrin induces angiogenesis in vascular endothelial cells through the Akt/endothelial nitric oxide synthase signaling pathway.
November 2015, Biochemical and biophysical research communications,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Serine 1179 phosphorylation of endothelial nitric oxide synthase caused by 2,4,6-trinitrotoluene through PI3K/Akt signaling in endothelial cells.
July 2006, Toxicology and applied pharmacology,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Induction of endothelial nitric oxide synthase, SIRT1, and catalase by statins inhibits endothelial senescence through the Akt pathway.
November 2010, Arteriosclerosis, thrombosis, and vascular biology,
Carmine Vecchione, and Angelo Maffei, and Salvatore Colella, and Alessandra Aretini, and Roberta Poulet, and Giacomo Frati, and Maria Teresa Gentile, and Luigi Fratta, and Valentina Trimarco, and Bruno Trimarco, and Giuseppe Lembo
Deferiprone increases endothelial nitric oxide synthase phosphorylation and nitric oxide production.
September 2018, Canadian journal of physiology and pharmacology,
Copied contents to your clipboard!