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The method of preparative isotachophoresis in acrylamide gel ensuring a high yield of IgD and IgE with insignificant admixtures of IgG, etc. was used for the isolation of IgD and IgE from the blood sera of myeloma patients. As a result of immunization with these antigens, monospecific IgD and IgE antisera were obtained. These antisera, alongside with specific antibodies, contained antibodies to admixtures; the latter were eliminated by the method of immune absorbtion carried out with the use of a sorbent based on Sepharose activated with bromo-cyanogen and conjugated with normal human blood serum. Ig D antisera were also shown to contain antibodies to idiotypical IgD determinants located in the Fab fragment of this immunoglobulin.
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
August 1977,
Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 1976,
Scandinavian journal of immunology,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 1981,
Laboratornoe delo,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
May 1998,
Veterinary immunology and immunopathology,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
March 1989,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
April 1983,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 2008,
Methods in molecular biology (Clifton, N.J.),
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 1970,
Acta biologica et medica Germanica,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 1993,
International archives of allergy and immunology,
K G Stoev, and
R L Panurina, and
E M Gavrilova, and
K L Shakhanina
January 1976,
Immunology,
