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Glucose stimulates the uptake of 45Ca into beta-cell-rich pancreatic islets isolated from ob/ob-mice. The distribution of the incorporated radioactivity was analysed by labelling the organelles with 45Ca in their cellular environment. The radioactive content of the organelles was measured after homogenization and fractionation of the islets under conditions preventing 45Ca redistribution. The 45Ca taken up in response to glucose appeared essentially in the secretory granule fraction and in that enriched in mitochondria. Modification of the 45Ca loading procedure, involving reduction of the oxygen tension and incubation volume, resulted in the disappearance of the glucose effect on the mitochondrial fraction whereas part of the stimulatory effect on the secretory granules persisted. Buffering of calcium by the secretory granules and mitochondria may be important for regulating the cytoplasmic Ca2+ involved in stimulus-secretion coupling.
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
January 1978,
Biochimica et biophysica acta,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
April 1979,
Acta endocrinologica,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
October 1980,
Biochemical medicine,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
January 1981,
Acta endocrinologica,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
October 1964,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
December 1981,
Biochemical medicine,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
November 1983,
The American journal of physiology,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
July 1978,
Biochimica et biophysica acta,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
July 1979,
FEBS letters,
K D Kohnert, and
H J Hahn, and
E Gylfe, and
H Borg, and
B Hellman
July 1980,
Biochimica et biophysica acta,
