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The first application of deuterium magentic resonance of specifically labelled lipids to the study of a natural biological membrane is described. Palmitic acid labelled at the terminal methyl group with deuterium was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii. The deuterium nuclear magnetic resonance spectra contain quadrupole splittings which yield directly order parameters for this region of the membrane. Below the growth temperature (37 degrees C) the spectra are indicative of lipid in both gel and liquid crystalline states. Above this temperature they demonstrate the existence of an entirely liquid crystalline membrane whose order parameter decreases rapidly with increasing temperature. Comparison with egg phosphatidylcholine over the same temperature range shows a more rapid change in order with temperature for the A. laidlawii membranes.
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
August 1980,
Biochimica et biophysica acta,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
January 1983,
The Yale journal of biology and medicine,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
February 1976,
Biochimica et biophysica acta,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
December 1972,
Journal of ultrastructure research,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
October 1992,
Biochemistry,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
April 1980,
Biochimica et biophysica acta,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
October 1984,
Lipids,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
November 1978,
Archives of biochemistry and biophysics,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
August 1980,
Biochemistry,
G W Stockton, and
K G Johnson, and
K W Butler, and
C F Polnaszek, and
R Cyr, and
I C Smith
September 1984,
FEBS letters,
