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G-banding of human pachytene bivalents is obtained using trypsin digestion. Each of the 22 autosomal bivalents is clearly identified. Such an identification leads to construction of the human pachytene map. Our results show the equivalence between the G-band patterns of mitotic chromosomes and meiotic bivalents. The G-band patterns of mitotic-meiotic chromosomes also correlate closely with the chromomere sequence of pachytene bivalents. Thus, study of chromomeres should constitute an excellent approach to comprehension of G-banding mechanism.
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