Effects of pravastatin on superoxide and fibronectin production of mesangial cells induced by low-density lipoprotein. 2002

Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Pravastatin is a potent inhibitor of HMG-CoA reductase and is effective in lowering serum lipid levels. Recent studies have shown that pravastatin also reduces the oxidative modification of low-density lipoprotein (LDL). To determine whether pravastatin has a direct effect on glomerular mesangial cells, we have measured the effects of pravastatin on the production of superoxide and fibronectin of glomerular mesangial cells stimulated by LDL. Our results demonstrated that the superoxide production of mesangial cells increased after LDL stimulation (100 microg/ml for 4 h) and that the superoxide production was significantly suppressed by either superoxide dismutase (SOD; 500 U/ml for 36 h; p < 0.01) or pravastatin (80 microM for 36 h; p < 0.05). The production of fibronectin was also increased after LDL stimulation which was also significantly suppressed by either SOD (p < 0.01) or pravastatin (p < 0.01). SOD or pravastatin alone had no effect on the unstimulated cells. Our results indicate that pravastatin may have a direct effect as an antioxidant and suppresses the fibronectin synthesis of glomerular mesangial cells independent of its hypolipidemic effect.

UI MeSH Term Description Entries
D008077 Lipoproteins, LDL A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues. Low-Density Lipoprotein,Low-Density Lipoproteins,beta-Lipoprotein,beta-Lipoproteins,LDL(1),LDL(2),LDL-1,LDL-2,LDL1,LDL2,Low-Density Lipoprotein 1,Low-Density Lipoprotein 2,LDL Lipoproteins,Lipoprotein, Low-Density,Lipoproteins, Low-Density,Low Density Lipoprotein,Low Density Lipoprotein 1,Low Density Lipoprotein 2,Low Density Lipoproteins,beta Lipoprotein,beta Lipoproteins
D008297 Male Males
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005353 Fibronectins Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins. Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D005920 Glomerular Mesangium The thin membranous structure supporting the adjoining glomerular capillaries. It is composed of GLOMERULAR MESANGIAL CELLS and their EXTRACELLULAR MATRIX. Mesangium, Glomerular,Mesangial Extracellular Matrix,Extracellular Matrices, Mesangial,Extracellular Matrix, Mesangial,Glomerular Mesangiums,Matrices, Mesangial Extracellular,Matrix, Mesangial Extracellular,Mesangial Extracellular Matrices,Mesangiums, Glomerular
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013481 Superoxides Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides. Superoxide Radical,Superoxide,Superoxide Anion
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
January 1994, Nephron,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
January 1999, Kidney international,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
July 1993, Atherosclerosis,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
December 1996, Journal of investigative medicine : the official publication of the American Federation for Clinical Research,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
December 1995, FEBS letters,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
January 2008, Nephron. Physiology,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
January 1999, Nephron,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
May 1989, Kidney international,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
July 2014, Biochemical and biophysical research communications,
Hung-Chun Chen, and Jinn-Yuh Guh, and Shyi-Jang Shin, and Yasuhiko Tomino, and Yung-Hsiung Lai
June 1994, Kidney international,
Copied contents to your clipboard!