BIOMAT Database Logo BIOMAT Database Logo

Protein kinase D is a downstream target of protein kinase Ctheta. 2002

Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Unit of Signal Transduction and Gastrointestinal Cancer, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA.

Protein kinase D (PKD/PKCmu immunoprecipitated from either COS-7 cells or Jurkat T lymphocytes transiently transfected with a constitutively active mutant of PKCtheta AE (PKCthetaAE) exhibited a marked increase in basal activity. In contrast, coexpression of constitutively active mutant of PKCzeta does not induce PKD activation in both types of cells. PKCthetaAE does not induce kinase activity in immunocomplexes of PKD kinase-deficient mutants PKDK618N or PKDD733A. PKD activation in response to PKCthetaAE signaling was completely prevented by treatment with the protein kinase C (PKC) inhibitors, GF I or Ro 31-8220, or by mutation of Ser-744 and Ser-748 to Ala in the kinase activation loop of PKD. Our results show that PKD is a downstream target of the theta isoform of PKC in both COS-7 cells and lymphocytes. The regulation of PKD by PKCtheta reveals a new pathway in the signaling network existing between multiple members of the PKC superfamily and PKD.

UI MeSH Term Description Entries
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008301 Maleimides Derivatives of maleimide (the structural formula H2C2(CO)2NH) containing a pyrroledione ring where the hydrogen atom of the NH group is replaced with aliphatic or aromatic groups.
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D011233 Precipitin Tests Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate. Precipitin Test,Test, Precipitin,Tests, Precipitin
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004791 Enzyme Inhibitors Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000074121 Protein Kinase C-theta A calcium-independent, phospholipid- and diacylglycerol-dependent, protein kinase C subtype that contains an N-terminal C2 DOMAIN and two diacylglycerol-binding ZINC FINGERS. It is expressed primarily by T-LYMPHOCYTES and localizes to IMMUNOLOGICAL SYNAPSES where it regulates downstream signaling for the activation, proliferation, and survival of mature T-cells. It plays a critical role in allergic, autoimmune, and alloimmune responses of TH2 CELLS and TH17 CELLS. PKC-theta,PRKCQ,Protein Kinase C theta,Protein Kinase C, theta,PKC theta

Related Publications

Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase D. A selective target for antigen receptors and a downstream target for protein kinase C in lymphocytes.
June 2000, The Journal of experimental medicine,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta is a specific target for inhibition of the HIV type 1 replication in CD4+ T lymphocytes.
August 2011, The Journal of biological chemistry,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of glycoprotein VI in platelets.
June 2010, The Journal of biological chemistry,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
CD4+ and CD8+ T cell survival is regulated differentially by protein kinase Ctheta, c-Rel, and protein kinase B.
March 2007, Journal of immunology (Baltimore, Md. : 1950),
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta expression is increased upon differentiation of human skeletal muscle cells: dysregulation in type 2 diabetic patients and a possible role for protein kinase Ctheta in insulin-stimulated glycogen synthase activity.
August 2000, Endocrinology,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta controls Th1 cells in experimental autoimmune encephalomyelitis.
December 2005, Journal of immunology (Baltimore, Md. : 1950),
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta: a new essential superstar on the T-cell stage.
November 2000, Immunology today,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Protein kinase Ctheta is required for autophagy in response to stress in the endoplasmic reticulum.
May 2008, The Journal of biological chemistry,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
P2X7 receptors activate protein kinase D and p42/p44 mitogen-activated protein kinase (MAPK) downstream of protein kinase C.
September 2002, The Biochemical journal,
Jingzhen Yuan, and David Bae, and Doreen Cantrell, and Andre E Nel, and Enrique Rozengurt
Mechanism of diacylglycerol-induced membrane targeting and activation of protein kinase Ctheta.
July 2007, The Journal of biological chemistry,
Copied contents to your clipboard!