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Ethacrynic acid, a loop diuretic drug, caused lipid peroxidation in isolated rat hepatocytes. The thiobarbituric acid reactive substances (TBARS) formation showed a good correlation with the leakage of glutamic-oxaloacetic acid transaminase (GOT) from the hepatocytes. The addition of antioxidants such as N, N'-diphenyl-p-phenylenediamine (DPPD) and promethazine to the isolated rat hepatocyte suspension containing ethacrynic acid prevented the lipid peroxidation and decreased the GOT leakage to some extent. SKF-525A inhibited the oxidative metabolism of ethacrynic acid and decreased the TBARS formation, suggesting that the lipid peroxidation was caused by the oxidative metabolism. The intracellular reduced glutathione markedly decreased in the hepatocyte suspension containing ethacrynic acid and the hepatocellular protein sulfhydryls were decreased, which was negatively correlated with the GOT leakage. Thus the ethacrynic acid-induced hepatotoxicity was found to be related to the lipid peroxidation and the decrease of cellular protein sulfhydryls.
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
August 2007,
Archives of toxicology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
April 1981,
Toxicology and applied pharmacology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
December 1998,
Toxicology in vitro : an international journal published in association with BIBRA,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
July 1984,
Biochemical pharmacology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
September 1986,
Toxicology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
May 2000,
Phytotherapy research : PTR,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
February 1975,
Biochemical pharmacology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
July 1993,
Toxicology letters,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
January 1978,
Anesthesiology,
K Yamamoto, and
Y Masubuchi, and
S Narimatsu, and
S Kobayashi, and
T Horie
June 1994,
The Journal of pharmacology and experimental therapeutics,
