Polyethylene glycol, determined by near-infrared reflectance spectroscopy, as a marker of fecal output in goats. 2002

S Landau, and S Friedman, and L Devash, and S J Mabjeesh
Institute of Field and Garden Crops, Department of Natural Resources, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel. vclandau@agri.gov.il

We report the application of NIR spectroscopy to determine the fecal concentration of poly(ethylene glycol) (PEG, MW 6000) used as an external marker of fecal output in goats. Calibration was carried out, using the modified partial least-squares method (MPLS), combining all wavelengths in the 1100-2500 nm range, with high linearity (R2 = 0.99). In goats fed at maintenance level, the recovery of PEG in feces was complete, and the estimation of fecal output was accurate, when a moderate dose of PEG was given (20 g/d). A higher dose of PEG (40 g/d) was associated with underestimation of fecal output, probably because PEG interacted with water metabolism. Using PEG and its NIRS-aided analysis to determine fecal output is accurate, simple, and cheap. However, the feasibility of this new method must be verified in goats feeding on tannin-containing diets, and in goats at high feeding level.

UI MeSH Term Description Entries
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006041 Goats Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP. Capra,Capras,Goat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000824 Animal Nutritional Physiological Phenomena Nutritional physiology of animals. Animal Nutrition Physiology,Animal Nutritional Physiology Phenomena,Animal Nutritional Physiological Phenomenon,Animal Nutritional Physiology,Animal Nutritional Physiology Phenomenon,Veterinary Nutritional Physiology,Nutrition Physiologies, Animal,Nutrition Physiology, Animal,Nutritional Physiology, Animal,Nutritional Physiology, Veterinary,Physiology, Animal Nutrition,Physiology, Animal Nutritional,Physiology, Veterinary Nutritional
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D019265 Spectroscopy, Near-Infrared A noninvasive technique that uses the differential absorption properties of hemoglobin and myoglobin to evaluate tissue oxygenation and indirectly can measure regional hemodynamics and blood flow. Near-infrared light (NIR) can propagate through tissues and at particular wavelengths is differentially absorbed by oxygenated vs. deoxygenated forms of hemoglobin and myoglobin. Illumination of intact tissue with NIR allows qualitative assessment of changes in the tissue concentration of these molecules. The analysis is also used to determine body composition. NIR Spectroscopy,Spectrometry, Near-Infrared,NIR Spectroscopies,Near-Infrared Spectrometries,Near-Infrared Spectrometry,Near-Infrared Spectroscopies,Near-Infrared Spectroscopy,Spectrometries, Near-Infrared,Spectrometry, Near Infrared,Spectroscopies, NIR,Spectroscopies, Near-Infrared,Spectroscopy, NIR,Spectroscopy, Near Infrared

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