Reverse passive haemagglutination test for the rapid identification of Neisseria gonorrhoeae and detection of penicillinase production. 1979

R Munro, and R Mallon

In a comparison of the reverse passive haemagglutination test (RPHA) with the direct immunofluorescent and rapid carbohydrate utilisation tests for the identification of Neisseria gonorrhoeae isolated from clinical specimens, 315 isolates of oxidase-positive Gram-negative diplococci were tested as pure 24-hour-old subcultures and samples from 108 similar organisms were taken directly from primary isolation plates. A similar test system was used to detect penicillinase production. Results showed agreement in 97.8% of organisms tested with the RPHA and conventional methods for identification of N. gonorrhoeae; similarly there was good agreement with conventional methods for detection of penicillinase production. The test was reliable and could be read within four hours; a result was therefore available on the same day the clinical specimen was received. The time and work involved in identifying N. gonorrhoeae using the RPHA was less than with conventional methods, but differentiation between N. gonorrhoeae and other Neisseria species from throat swabs proved difficult.

UI MeSH Term Description Entries
D008297 Male Males
D009344 Neisseria gonorrhoeae A species of gram-negative, aerobic bacteria primarily found in purulent venereal discharges. It is the causative agent of GONORRHEA. Diplococcus gonorrhoeae,Gonococcus,Gonococcus neisseri,Merismopedia gonorrhoeae,Micrococcus der gonorrhoe,Micrococcus gonococcus,Micrococcus gonorrhoeae
D010405 Penicillinase A beta-lactamase preferentially cleaving penicillins. (Dorland, 28th ed) EC 3.5.2.-. beta-Lactamase I,AER-I beta-Lactamase,Benzylpenicillinase,Carbenicillinase,Exopenicillinase,beta Lactamase III,beta Lactamase RP4,gamma-Penicillinase,AER I beta Lactamase,Lactamase RP4, beta,beta Lactamase I,beta-Lactamase, AER-I,gamma Penicillinase
D005260 Female Females
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006069 Gonorrhea Acute infectious disease characterized by primary invasion of the urogenital tract. The etiologic agent, NEISSERIA GONORRHOEAE, was isolated by Neisser in 1879. Neisseria gonorrhoeae Infection
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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