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Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate: evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosus. 2002

Jan Massant, and Patrik Verstreken, and Virginie Durbecq, and Abdelaziz Kholti, and Christianne Legrain, and Sonia Beeckmans, and Pierre Cornelis, and Nicolas Glansdorff
Department of Microbiology, Vrije Universiteit Brussel, Flanders Interuniversity Institute for Biotechnology, Université Libre de Bruxelles, Belgium.

Two different approaches provided evidence for a physical interaction between the carbamate kinase-like carbamoyl-phosphate synthetase (CKase) and ornithine carbamoyltransferase (OTCase) from the hyperthermophilic archaeon Pyrococcus furiosus. Affinity electrophoresis indicated that CKase and OTCase associate into a multienzyme cluster. Further evidence for a biologically significant interaction between CKase and OTCase was obtained by co-immunoprecipitation combined with formaldehyde cross-linking experiments. These experiments support the hypothesis that CKase and OTCase form an efficient channeling cluster for carbamoyl phosphate, an extremely thermolabile and potentially toxic metabolic intermediate. Therefore, by physically interacting with each other, CKase and OTCase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment.

UI MeSH Term Description Entries
D009954 Ornithine Carbamoyltransferase A urea cycle enzyme that catalyzes the formation of orthophosphate and L-citrulline (CITRULLINE) from CARBAMOYL PHOSPHATE and L-ornithine (ORNITHINE). Deficiency of this enzyme may be transmitted as an X-linked trait. EC 2.1.3.3. Ornithine Transcarbamylase,Ornithine Carbamylphosphate Transferase,Carbamoyltransferase, Ornithine,Carbamylphosphate Transferase, Ornithine,Transcarbamylase, Ornithine,Transferase, Ornithine Carbamylphosphate
D011233 Precipitin Tests Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate. Precipitin Test,Test, Precipitin,Tests, Precipitin
D002221 Carbamyl Phosphate The monoanhydride of carbamic acid with PHOSPHORIC ACID. It is an important intermediate metabolite and is synthesized enzymatically by CARBAMYL-PHOSPHATE SYNTHASE (AMMONIA) and CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING). Carbamoyl Phosphate,Dilithium Carbamyl Phosphate,Carbamyl Phosphate, Dilithium,Phosphate, Carbamoyl,Phosphate, Carbamyl,Phosphate, Dilithium Carbamyl
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D017851 Phosphotransferases (Carboxyl Group Acceptor) A class of enzymes that transfers phosphate groups and has a carboxyl group as an acceptor. EC 2.7.2.
D019731 Carbon-Nitrogen Ligases Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3. Carbon Nitrogen Ligases,Ligases, Carbon-Nitrogen
D020027 Pyrococcus furiosus A species of strictly anaerobic, hyperthermophilic archaea which lives in geothermally-heated marine sediments. It exhibits heterotropic growth by fermentation or sulfur respiration.

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