BIOMAT Database Logo BIOMAT Database Logo

[The enzymatic hydrolysis of cell wall applied to yeasts classification (author's transl)]. 1975

M Bastide, and P Trave, and J M Bastide

The rigid structure of yeast cell wall may be a taxonomic criterion. Using the Helix pomatia juice (with mercaptoethylamine hydrochloride) as a polyenzymatic system, we have always obtained protoplasts from ascomycetous yeasts and never from basidiomycetous yeasts tested. Then, we have applied this enzymatic analysis to the Fungi imperfecti yeasts and, according to this test, separated genus looking like basidiomycetous behaviour: Trichosporon, Pityrosporum, Rhodotorula, Cryptococcus and non fermentative Candida (group II). Oppositely, we have put together the Fungi imperfecti yeasts looking like ascomycetous behaviour: Kloeckera, Trigonopsis, Brettanomyces and fermentative Candida (group I). With the second lytic system made of mercaptoethylamine-HCl and beta-(1-3)-D-glucanase, we have selected ascomycetous yeasts giving protoplasts: Saccharomycoidea with genus Saccharomyces, Kluyveromyces, Pichia, Hansenula, Citeromyces, Debaryomyces; we have joined Saccharomycodes and Hanseniaspora to this second group. The other ascomycetous yeasts studied did not give protoplasts with the definite lytic system made of purified beta-(1-3)-D-glucanase and thiol. These results seem to demonstrate that the structure of cell wall can act as an important taxonomic criterion.

UI MeSH Term Description Entries
D011523 Protoplasts The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL. Protoplast
D002473 Cell Wall The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents. Cell Walls,Wall, Cell,Walls, Cell
D003562 Cytidine A pyrimidine nucleoside that is composed of the base CYTOSINE linked to the five-carbon sugar D-RIBOSE. Cytosine Ribonucleoside,Cytosine Riboside,Ribonucleoside, Cytosine,Riboside, Cytosine
D003904 Mitosporic Fungi A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group. Deuteromycetes,Deuteromycota,Fungi imperfecti,Fungi, Mitosporic,Hyphomycetes,Deuteromycete,Deuteromycotas,Fungi imperfectus,Fungus, Mitosporic,Hyphomycete,Mitosporic Fungus,imperfectus, Fungi
D004693 Glucan Endo-1,3-beta-D-Glucosidase An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman. Endo-1,3-beta-Glucanase,Oligo-1,3-Glucosidase,Zymolase,Endo 1,3 beta Glucanase,Endo-1,3-beta-D-Glucosidase, Glucan,Glucan Endo 1,3 beta D Glucosidase,Oligo 1,3 Glucosidase
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006151 Guanosine A purine nucleoside that has guanine linked by its N9 nitrogen to the C1 carbon of ribose. It is a component of ribonucleic acid and its nucleotides play important roles in metabolism. (From Dorland, 28th ed)
D006372 Helix, Snails A genus of chiefly Eurasian and African land snails including the principal edible snails as well as several pests of cultivated plants. Helix (Snails),Snails Helix
D001203 Ascomycota A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum. Ascomycetes,Cochliobolus,Sclerotinia,Ascomycete,Ascomycotas,Sclerotinias
D001487 Basidiomycota A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi. Basidiomycetes,Basidiomycete,Basidiomycotas

Related Publications

M Bastide, and P Trave, and J M Bastide
[The lysis of the cell wall of yeasts (author's transl)].
January 1974, Zeitschrift fur allgemeine Mikrobiologie,
M Bastide, and P Trave, and J M Bastide
The mechanisms of plant cell wall deconstruction during enzymatic hydrolysis.
January 2014, PloS one,
M Bastide, and P Trave, and J M Bastide
[Enzymatic hydrolysis of sutures of the cavity after Hryntschak prostatectomy (author's transl)].
January 1973, Urologia internationalis,
M Bastide, and P Trave, and J M Bastide
[Enzymatic hydrolysis of Ginseng saponins and their related glycosides (author's transl)].
February 1975, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
M Bastide, and P Trave, and J M Bastide
[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].
April 1975, Zeitschrift fur klinische Chemie und klinische Biochemie,
M Bastide, and P Trave, and J M Bastide
[The arrangement of wall-polymers in staphylococcal cell wall (author's transl)].
June 1980, Fukuoka igaku zasshi = Hukuoka acta medica,
M Bastide, and P Trave, and J M Bastide
[Kininases from yeasts (author's transl)].
January 1975, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
M Bastide, and P Trave, and J M Bastide
CELL WALL OF YEASTS.
January 1963, Annual review of microbiology,
M Bastide, and P Trave, and J M Bastide
[Radioimmunological differentiation of yeasts (author's transl)].
October 1978, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
M Bastide, and P Trave, and J M Bastide
[Evaluation of enzymatic activities of yeasts in epidemiological investigations of urogenital yeast infections (author's transl)].
May 1980, Dermatologische Monatschrift,
Copied contents to your clipboard!