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[Cloning of the human cytochrome p450 2B6 gene in Escherichia coli cells]. 2001
S N Kolomeĭchuk, and
M V Pokrovskaia, and
A A Borisova, and
A A Zhgun, and
L I Solodar', and
N N Sokolov, and
V I Shvets, and
Iu V Gervaziev, and
M A El'darov, and
A I Archakov
Orekhovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow 119992.
Human cytochrome P450 2B6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+)/CYP 2B6 was expressed in E. coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium.
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